PMID: 97666May 1, 1978

Uracil incorporation into nascent DNA of thymine-requiring mutant of Bacillus subtilis 168

Proceedings of the National Academy of Sciences of the United States of America
F Tamanoi, T Okazaki

Abstract

A thymine-requiring mutant of Bacillus subtilis strain 168 accumulates short DNA chains after brief pulses with [(3)H]thymidine. Reversion of the thy mutation to thy(+) abolishes the accumulation of short DNA chains, suggesting that the accumulation is related to the thy mutation. The reason for this accumulation has been further investigated by analysis of a mutant with a defective uracil-DNA glycosidase activity (urg). The accumulation of short DNA chains in thy(-) cells is abolished by the deficiency of uracil-DNA glycosidase activity. In thy(+) cells, the deficiency of the glycosidase activity does not change the sedimentation profile of pulse-labeled DNA. DNA isolated from thy(-)urg(-) cells is fragmented by successive treatment with purified uracil-DNA glycosidase and alkali, indicating that uracil residues are present in this DNA. DNA isolated from thy(+)urg(-) cells is not fragmented by the same treatment. Significant radioactivity is detected in the dUMP region, when [(3)H]uridine-labeled DNA from thy(-)urg(-) cells is hydrolyzed and analyzed by thin-layer chromatography. Only a trace amount of radioactivity, which is not influenced by the deficiency of uracil-DNA glycosidase activity, is found in the dUMP region in DN...Continue Reading

References

Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B K TyeB Weiss
Jul 11, 1977·Biochemical and Biophysical Research Communications·F TamanoiR Okazaki
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Citations

May 23, 1979·Molecular & General Genetics : MGG·T J KwohM H Patrick
Jan 1, 1983·Molecular & General Genetics : MGG·R J Melamede, S S Wallace
Jan 1, 1984·Advances in Enzyme Regulation·R G RichardsW D Sedwick
Oct 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Gemma Serrano-HerasMargarita Salas
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M GoulianB Y Tseng
Oct 20, 2009·Journal of Bacteriology·Luciana Amado, Andrei Kuzminov
Aug 31, 2012·Microbiology and Molecular Biology Reviews : MMBR·Justin S LenhartLyle A Simmons
Sep 1, 1995·Medical Hypotheses·E Jennings
Apr 1, 1986·Experimental Cell Research·M M Hurt, R E Moses
Mar 30, 1979·Biochemical and Biophysical Research Communications·H Ariga, H Shimojo
Mar 28, 1986·Biochemical and Biophysical Research Communications·D C Fraser, C K Pearson
Mar 5, 1990·Journal of Molecular Biology·M Sassanfar, J W Roberts
Jun 15, 2006·The Journal of Biological Chemistry·Luciana Amado, Andrei Kuzminov
Jun 1, 1985·Microbiological Reviews·P J Piggot, J A Hoch
Jan 1, 1982·Environmental Mutagenesis·B A Kunz
Jan 9, 2019·Proceedings of the National Academy of Sciences of the United States of America·Glen E CronanAndrei Kuzminov

Related Concepts

Natto Bacteria
Deoxyuracil Nucleotides
Base Excision Repair
DNA Replication
DNA, Bacterial
Nucleosidases
Thymine
Uracil

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