Uracil recognition by archaeal family B DNA polymerases

Biochemical Society Transactions
B A ConnollyB T Wilson

Abstract

Archaeal family-B DNA polymerases possess a novel uracil-sensing mechanism. A specialized pocket scans the template, ahead of the replication fork, for the presence of uracil; on encountering this base, DNA synthesis is stalled. The structural basis for uracil recognition by polymerases is described and compared with other uracil-recognizing enzymes (uridine-triphosphate pyrophophatases and uracil-DNA glycosylases). Remarkably, protein-protein interactions between all three archaeal uracil sensors are observed; possibly the enzymes co-operate to efficiently eliminate uracil from archaeal genomes.

Citations

May 3, 2014·Archaea : an International Microbiological Journal·Felipe SarmientoWilliam B Whitman
Apr 1, 2014·Nucleic Acids Research·Benjamin A KeenAidan J Doherty
Jul 21, 2004·Computational Biology and Chemistry·Scott E WhitneyHendrik J Viljoen
Aug 26, 2015·Nucleic Acids Research·Pau Biak SangUmesh Varshney
May 7, 2016·Scientific Reports·Miyako ShiraishiYoshizumi Ishino
Oct 8, 2011·Critical Reviews in Biochemistry and Molecular Biology·Alan J HerrBradley D Preston
Jan 16, 2009·Biochemical Society Transactions·Shinichi KiyonariYoshizumi Ishino
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Elizabeth R Barry, Stephen D Bell
Apr 19, 2005·Computational Biology and Chemistry·Steve ViljoenHendrik Viljoen
Sep 7, 2021·Chembiochem : a European Journal of Chemical Biology·Heike M KroppAndreas Marx

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