PMID: 2503811Jul 11, 1989Paper

Uranyl mediated photofootprinting reveals strong E. coli RNA polymerase--DNA backbone contacts in the +10 region of the DeoP1 promoter open complex

Nucleic Acids Research
C Jeppesen, P E Nielsen

Abstract

Employing a newly developed uranyl photofootprinting technique (Nielsen et al. (1988) FEBS Lett. 235, 122), we have analyzed the structure of the E. coli RNA polymerase deoP1 promoter open complex. The results show strong polymerase DNA backbone contacts in the -40, -10, and most notably in the +10 region. These results suggest that unwinding of the -12 to +3 region of the promoter in the open complex is mediated through polymerase DNA backbone contacts on both sides of this region. The pattern of bases that are hyperreactive towards KMnO4 or uranyl within the -12 to +3 region furthermore argues against a model in which this region is simply unwound and/or single stranded. The results indicate specific protein contacts and/or a fixed DNA conformation within the -12 to +3 region.

References

Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T D Tullius, B A Dombroski
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J Ramstein, R Lavery
Jan 1, 1987·CRC Critical Reviews in Biochemistry·A A Travers
Feb 29, 1988·FEBS Letters·W P ZhenP E Nielsen
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Oct 24, 1980·Nucleic Acids Research·C M Rubin, C W Schmid

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Citations

May 1, 1996·Journal of Molecular Recognition : JMR·P E Nielsen, N E Møllegaard
Oct 1, 1991·Journal of Biomolecular Structure & Dynamics·R Beger, E W Prohofsky
Jun 11, 1992·Nucleic Acids Research·P E Nielsen
Apr 25, 1993·Nucleic Acids Research·E L LempersN M Kostić
Feb 13, 2001·Nucleic Acids Research·V StuditskyA Mirzabekov
Mar 1, 1991·Photochemistry and Photobiology·U HenriksenO Buchardt
Nov 1, 1989·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P E Nielsen
Jun 30, 2000·Journal of Molecular Biology·D WittbergerR Schroeder
Nov 11, 1991·Bioconjugate Chemistry·A R Hill, L E Orgel
Oct 2, 1990·Biochemistry·D S Sigman
Jun 10, 2017·Chemical Reviews·Wenhu ZhouJuewen Liu
Sep 15, 1991·Physical Review. a·R Beger, E W Prohofsky
Feb 1, 1995·Journal of Biomolecular Structure & Dynamics·C Bailly, M J Waring
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·S E Rokita
Feb 1, 1990·Journal of Molecular Recognition : JMR·P E Nielsen
Sep 22, 2021·Chemical Reviews·Vivian M LechnerMatthew J Gaunt

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