Urban metagenomics uncover antibiotic resistance reservoirs in coastal beach and sewage waters

Microbiome
Pablo FresiaGregorio Iraola

Abstract

Microbial communities present in environmental waters constitute a reservoir for antibiotic-resistant pathogens that impact human health. For this reason, a diverse variety of water environments are being analyzed using metagenomics to uncover public health threats. However, the composition of these communities along the coastal environment of a whole city, where sewage and beach waters are mixed, is poorly understood. We shotgun-sequenced 20 coastal areas from the city of Montevideo (capital of Uruguay) including beach and sewage water samples to characterize bacterial communities and their virulence and antibiotic resistance repertories. As expected, we found that sewage and beach environments present significantly different bacterial communities. This baseline allowed us to detect a higher prevalence and a more diverse repertory of virulence and antibiotic-resistant genes in sewage samples. Many of these genes come from well-known enterobacteria and represent carbapenemases and extended-spectrum betalactamases reported in hospital infections in Montevideo. Additionally, we were able to genotype the presence of both globally disseminated pathogenic clones and emerging antibiotic-resistant bacteria in sewage waters. Our study ...Continue Reading

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Citations

Jan 31, 2020·Environmental Microbiology·Vincent Hervé, Pascal Jean Lopez
Jul 5, 2019·Clinical Microbiology Reviews·Daniela Costa, Gregorio Iraola
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Jun 20, 2021·Letters in Applied Microbiology·A L B CanellasM S Laport

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Datasets Mentioned

BETA
PRJNA515946
AAC
38424
55407.2

Methods Mentioned

BETA
phosphotransferases
PCR

Software Mentioned

LEfSe
Trimmomatic
FastQC
Vegan R package
EST
MEGAHIT
MAFFT
R package
Minimus2
+ blastx

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

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