Urban traffic-derived nanoparticulate matter reduces neurite outgrowth via TNFα in vitro

Journal of Neuroinflammation
Hank ChengC E Finch

Abstract

The basis for air pollution-associated neurodegenerative changes in humans is being studied in rodent models. We and others find that the ultrafine particulate matter (PM) derived from vehicular exhaust can induce synaptic dysfunction and inflammatory responses in vivo and in vitro. In particular, a nano-sized subfraction of particulate matter (nPM, PM0.2) from a local urban traffic corridor can induce glial TNFα production in mixed glia (astrocytes and microglia) derived from neonatal rat cerebral cortex. Here, we examine the role of TNFα in neurite dysfunctions induced by nPM in aqueous suspensions at 12 μg/ml. First, we show that the proximal brain gateway to nPM, the olfactory neuroepithelium (OE), rapidly responds to nPM ex vivo, with induction of TNFα, activation of macrophages, and dendritic shrinkage. Cell interactions were further analyzed with mixed glia and neurons from neonatal rat cerebral cortex. Microglia contributed more than astrocytes to TNFα induction by nPM. We then showed that the threefold higher TNFα in conditioned media (nPM-CM) from mixed glia was responsible for the inhibition of neurite outgrowth by small interfering RNA (siRNA) TNFα knockdown and by TNFα immunoneutralization. Despite lack of TNFR1 in...Continue Reading

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Citations

Sep 22, 2016·Frontiers in Neuroendocrinology·Mariana F UchoaChristian J Pike
Dec 3, 2016·Particle and Fibre Toxicology·Christina R TylerMatthew J Campen
Apr 16, 2017·Journal of Neuroinflammation·Nicholas C WoodwardCaleb E Finch
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Oct 26, 2020·Environmental Research·Heather E VolkUNKNOWN Environmental influences on Child Health Outcomes
May 11, 2021·Journal of Alzheimer's Disease : JAD·Hongqiao ZhangCaleb E Finch
Aug 17, 2021·Frontiers in Cell and Developmental Biology·Kai YouXuewen Xu
Aug 26, 2021·Journal of Neurodevelopmental Disorders·Amin HaghaniHeather E Volk

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Methods Mentioned

BETA
biopsies
transfection
PCR
GTPase

Software Mentioned

NeuronJ
ImageJ

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