Abstract
Calcium/calmodulin-dependent protein kinase IV (CaMKIV) is a multifunctional enzyme which belongs to the Ser/Thr kinase family. CaMKIV plays important role in varieties of biological processes such as gene expression regulation, memory consolidation, bone growth, T-cell maturation, sperm motility, regulation of microtubule dynamics, cell-cycle progression, and apoptosis. To measure stability parameters, urea-induced denaturation of CaMKIV was carried out at pH 7.4 and 25°C, using three different probes, namely far-UV CD, near-UV absorption, and tryptophan fluorescence. A coincidence of normalized denaturation curves of these optical properties suggests that urea-induced denaturation is a two-state process. Analysis of these denaturation curves gave values of 4.20 ± 0.12 kcal mol(-1), 2.95 ± 0.15 M, and 1.42 ± 0.06 kcal mol(-1) M(-1) for [Formula: see text] (Gibbs free energy change (ΔGD) in the absence of urea), Cm (molar urea concentration ([urea]) at the midpoint of the denaturation curve), and m (=∂ΔGD/∂[urea]), respectively. All these experimental observations have been fully supported by 30 ns molecular dynamics simulation studies.
References
Aug 1, 1991·Molecular and Cellular Biology·A R MeansT Ono
Oct 18, 1988·Biochemistry·M M Santoro, D W Bolen
Jan 1, 1986·Methods in Enzymology·C N Pace
Jan 1, 1968·Advances in Protein Chemistry·C Tanford
Feb 14, 1971·Journal of Molecular Biology·B Lee, F M Richards
Jul 1, 1967·Biochemistry·H Edelhoch
Aug 18, 1995·The Journal of Biological Chemistry·H TokumitsuT R Soderling
Jan 13, 1995·Cell·N RoyX Kang
May 16, 1994·Gene·M M BlandC A Ohmstede
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D T LooC W Cotman
Nov 1, 1995·Protein Science : a Publication of the Protein Society·C N PaceT Gray
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·H EnslenT R Soderling
Apr 8, 1998·Annual Review of Neuroscience·A J SilvaS Kida
Sep 4, 1998·Science·S ChawlaH Bading
Jun 15, 1999·Trends in Biochemical Sciences·T R Soderling
Jan 5, 2000·The Journal of Biological Chemistry·F BlaeserT A Chatila
Mar 14, 2000·The Journal of Biological Chemistry·J Y Wu, A R Means
Aug 10, 2000·Nature Genetics·J Y WuA R Means
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·S S Hook, A R Means
Apr 24, 2002·Neuron·Soren ImpeyRichard H Goodman
Jun 18, 2002·Cancer Letters·Noriyuki TakaiIsao Miyakawa
Jul 21, 2004·Progress in Biophysics and Molecular Biology·Herbert M Sauro, Boris N Kholodenko
Jan 14, 2005·Nature Reviews. Drug Discovery·Irena Melnikova, James Golden
May 15, 2007·The Journal of Physical Chemistry. B·Martin C Stumpe, Helmut Grubmüller
Mar 12, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Md Khurshid Alam KhanFaizan Ahmad
Sep 4, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tomohiro KogaGeorge C Tsokos
Jul 9, 2013·Journal of Computational Chemistry·Jing Huang, Alexander D MacKerell
Nov 21, 2013·Journal of Biomolecular Structure & Dynamics·Shah Ubaid-UllahFaizan Ahmad
Nov 28, 2013·Journal of Biomolecular Structure & Dynamics·Khalid AnwerMd Imtaiyaz Hassan
Apr 1, 2014·Current Protein & Peptide Science·Syed Ausaf AliFaizan Ahmad
Sep 2, 2014·Journal of Biomolecular Structure & Dynamics·Md Anzarul HaqueFaizan Ahmad
Oct 22, 2014·BMC Neuroscience·Lin LiuJun Li
Jan 15, 2015·Journal of Biomolecular Structure & Dynamics·Abdullah NaiyerFaizan Ahmad
May 2, 2015·Journal of Biomolecular Structure & Dynamics·Nasimul HodaB Jayaram
Sep 30, 2015·Gene·Faez Iqbal KhanMd Imtaiyaz Hassan
Oct 1, 2015·Journal of Biomolecular Structure & Dynamics·Danish IdreesMd Imtaiyaz Hassan
Jan 17, 2016·Progress in Biophysics and Molecular Biology·Huma NazMd Imtaiyaz Hassan
Jan 20, 2016·Bioorganic & Medicinal Chemistry Letters·Huma NazMd Imtaiyaz Hassan
Citations
Apr 20, 2016·Journal of Biomolecular Structure & Dynamics·Amresh PrakashMd Imtaiyaz Hassan
May 14, 2016·Journal of Biomolecular Structure & Dynamics·Parvez KhanMd Imtaiyaz Hassan
Nov 4, 2016·Chemical Biology & Drug Design·Ehtesham JameelMd Imtaiyaz Hassan
Jul 26, 2018·Journal of Biomolecular Structure & Dynamics·Anam BegMd Imtaiyaz Hassan
Dec 23, 2017·Journal of Biomolecular Structure & Dynamics·Sunayana Begum SyedMd Imtaiyaz Hassan
Apr 26, 2019·Journal of Cellular Biochemistry·Huma NazMd Imtaiyaz Hassan
May 29, 2019·Journal of Cellular Biochemistry·Ahmad Abu Turab NaqviImtaiyaz Hassan
Mar 7, 2020·Journal of Biomolecular Structure & Dynamics·Ahmad Abu Turab NaqviMd Imtaiyaz Hassan
Jul 15, 2020·Journal of Biomolecular Structure & Dynamics·Khalid A AlamryMd Imtaiyaz Hassan
Jul 30, 2020·Oxidative Medicine and Cellular Longevity·Preeti GuptaMd Imtaiyaz Hassan
Sep 10, 2019·Journal of Biomolecular Structure & Dynamics·Saman FatimaMd Imtaiyaz Hassan
Dec 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Taj MohammadImtaiyaz Hassan
Jul 27, 2017·Molecular and Cellular Biochemistry·Huma NazMd Imtaiyaz Hassan
Oct 16, 2020·Cell Biochemistry and Biophysics·Kehinde F OmolabiMahmoud E S Soliman