PMID: 7011372Feb 17, 1981Paper

Urea-induced unfolding of the alpha subunit of tryptophan synthase: evidence for a multistate process

Biochemistry
C R Matthews, M M Crisanti

Abstract

The urea-induced unfolding of the alpha subunit of tryptophan synthase from E. coli was monitored by optical spectroscopy and by urea-gradient gel electrophoresis. Three independent lines of evidence support the conclusion that one or more stable intermediates are present in this process: (i) Satisfactory fits of the equilibrium unfolding transitions obtained from difference spectroscopy at 286 nm and circular dichroism spectroscopy at 222 nm require a model which involves a stable intermediate in addition to the native and unfolded forms. (ii) Kinetic studies of the change in the extinction coefficient at 286 nm show that while the unfolding is well described by a single exponential change the refolding kinetics are complex. The nature of the dependence of the refolding kinetics on the initial concentration of urea supports the conclusion that at least one stable intermediate exists. (iii) The patterns obtained from urea-gradient gel electrophoresis experiments on the alpha subunit show that at least one and possibly two stable intermediates are involved; the intermediates have markedly different degrees of compactness. A kinetic model for the folding of the alpha subunit, consistent with all of these results, can be formulated.

Citations

Dec 1, 1993·Protein Science : a Publication of the Protein Society·J SafarC J Gibbs
Jun 1, 1993·Proteins·D T Haynie, E Freire
May 15, 1984·Journal of Molecular Biology·R HawkesJ Schellman
May 29, 1998·Biochimica Et Biophysica Acta·B VenkateshaH S Savithri
Dec 19, 2002·Biophysical Chemistry·Louise A Wallace, C Robert Matthews
Oct 1, 1996·Nature Biotechnology·J M Yon
Jun 24, 2000·European Journal of Biochemistry·H J Lee, G G Chang
Jul 1, 1999·Protein Science : a Publication of the Protein Society·J A ZitzewitzC R Matthews
Nov 2, 1987·FEBS Letters·R I Gilmanshin, O B Ptitsyn
Jun 5, 2007·Biochimica Et Biophysica Acta·T C JyothiA G Appu Rao
May 11, 2006·Proteins·Serapion PyrpassopoulosGeorge Nounesis
Apr 1, 1984·Analytical Biochemistry·D P Goldenberg, T E Creighton
Nov 10, 2004·Journal of Molecular Biology·Bryan A KrantzR John Collier
Jun 12, 2012·Journal of Molecular Biology·Pei-Fen Liu, Chiwook Park
Jul 30, 1985·Biochemistry·P W MuiH A Scheraga
Jul 21, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·N F Martins, M M Santoro

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