Urea transporters UT-A1 and UT-A3 accumulate in the plasma membrane in response to increased hypertonicity.

American Journal of Physiology. Renal Physiology
Nathan W BlessingJanet D Klein

Abstract

The UT-A1 and UT-A3 urea transporters are expressed in the terminal inner medullary collecting duct (IMCD) and play an important role in the production of concentrated urine. We showed that both hyperosmolarity and vasopressin increase urea permeability in perfused rat terminal IMCDs and that UT-A1 and UT-A3 accumulate in the plasma membrane in response to vasopressin. In this study, we investigated whether hyperosmolarity causes UT-A1 and/or UT-A3 to accumulate in the plasma membrane or represents a complimentary stimulatory pathway. Rat IMCD suspensions were incubated in 450 vs. 900 mosM solutions. We biotinylated the IMCD surface proteins, collected, and analyzed them. Membrane accumulation was assessed by Western blotting of the biotinylated protein pool probed with anti-UT-A1 or anti-UT-A3. We studied the effect of NaCl, urea, and sucrose as osmotic agents. Membrane-associated UT-A1 and UT-A3 increased relative to control levels when either NaCl (UT-A1 increased 37 +/- 6%; UT-A3 increased 46 +/- 13%) or sucrose (UT-A1 increased 81 +/- 13%; UT-A3 increased 60 +/- 8%) was used to increase osmolarity. There was no increase in membrane UT-A1 or UT-A3 when urea was added. Analogously, UT-A1 phosphorylation was increased in NaCl...Continue Reading

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Citations

Sep 26, 2012·Pflügers Archiv : European journal of physiology·Janet D KleinJeff M Sands
Sep 24, 2010·American Journal of Physiology. Renal Physiology·Yanhua WangJeff M Sands
Nov 20, 2013·Annual Review of Physiology·Jeff M Sands, Harold E Layton
Jun 16, 2009·Seminars in Nephrology·Jeff M Sands, Harold E Layton
Mar 15, 2016·Pflügers Archiv : European journal of physiology·Xiaoqian QianGuangping Chen
Apr 1, 2011·British Journal of Pharmacology·Gavin Stewart
Dec 10, 2014·Nature Reviews. Nephrology·Cristina Esteva-FontAlan S Verkman
May 14, 2016·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Lotte B BertelsenChristoffer Laustsen
Aug 1, 2014·Clinical Journal of the American Society of Nephrology : CJASN·I David WeinerJeff M Sands
Oct 20, 2017·American Journal of Physiology. Cell Physiology·Chung-Lin ChouMark A Knepper
Oct 3, 2020·Nature Reviews. Nephrology·Adriana MartonJens Titze
Aug 21, 2015·American Journal of Physiology. Renal Physiology·Chia Wei TeohDamien Gerard Noone
Oct 10, 2014·American Journal of Physiology. Renal Physiology·Huiwen RenBaoxue Yang

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