Nucleotide sequence analysis of a Ureaplasma urealyticum DNA fragment, homologous to cloned urease genes of other prokaryotes, revealed three consecutive open reading frames. The molecular weights of the three deduced polypeptides are 11.2 kD, 13.6 kD and 66.6 kD. These values are consistent with the size of the three subunits previously reported for purified native urease. A significant sequence homology was found between the three polypeptides of the ureaplasmal urease and the single polypeptide of jack bean (Canavalia ensiformis) urease. Codon usage indicates that UGA is a tryptophan codon in this mollicute. Use of polymerase chain reactions has disclosed the existence of genetic polymorphism among the urease genes of different serotypes of U. urealyticum.
Codon recognition patterns as deduced from sequences of the complete set of transfer RNA species in Mycoplasma capricolum. Resemblance to mitochondria
Cloning of Ureaplasma urealyticum DNA sequences showing genetic homology with urease genes from gram-negative bacteria
Purification, characterization, and genetic organization of recombinant Providencia stuartii urease expressed by Escherichia coli
Recovery of a soybean urease genomic clone by sequential library screening with two synthetic oligodeoxynucleotides
DNA cleavage patterns as indicators of genotypic heterogeneity among strains of Acholeplasma and Mycoplasma species
Higher prevalence of urogenital mycoplasmas in human immunodeficiency virus-positive patients as compared to patients with other sexually transmitted diseases
Comparison of synovial tissue and synovial fluid as the source of nucleic acids for detection of Chlamydia trachomatis by polymerase chain reaction
Site-directed mutagenesis of Klebsiella aerogenes urease: identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis
The application of epitope mapping in the development of a new serological test for Helicobacter pylori infection
Role of Ureaplasma urealyticum and Chlamydia trachomatis in development of bronchopulmonary dysplasia in very low birth weight infants
Growth inhibition of Ureaplasma urealyticum by the proton pump inhibitor lansoprazole: direct attribution to inhibition by lansoprazole of urease activity and urea-induced ATP synthesis in U. urealyticum.
Rapid detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum organisms in genitourinary samples by PCR-microtiter plate hybridization assay.
Biovar diversity of Ureaplasma urealyticum in amniotic fluid: distribution, intrauterine inflammatory response and pregnancy outcomes
Polymerase chain reaction-amplification of urease genes: rapid screening for ureaplasma urealyticum infection in endotracheal aspirates of ventilated newborns
Genetic code evolution reveals the neutral emergence of mutational robustness, and information as an evolutionary constraint
Detection and biovar discrimination of Ureaplasma urealyticum in Indian patients with genital tract infections
Development of real-time PCR for the differential detection and quantification of Ureaplasma urealyticum and Ureaplasma parvum
Detection of bacteria and analyses of Chlamydia trachomatis viability in patients with postvenereal reactive arthritis
Molecular methods for the detection of Mycoplasma and ureaplasma infections in humans: a paper from the 2011 William Beaumont Hospital Symposium on molecular pathology
Polymerase chain reaction as a sensitive and rapid method for specific detection of Mycoplasma pneumoniae in clinical samples
Relevant prevalence of Mycoplasma hominis and Ureaplasma urealyticum serogroups in HIV-1 infected men without urethritis symptoms
Identification of Mycoplasma hominis, Ureaplasma urealyticum, and Chlamydia trachomatis by polymerase chain reaction
Lack of protection against gastric Helicobacter infection following immunisation with jack bean urease: the rejection of a novel hypothesis
The number of domains in the ribosomal protein S1 as a hallmark of the phylogenetic grouping of bacteria
Molecular diagnosis of Ureaplasma urealyticum septic arthritis in a patient with hypogammaglobulinemia
Prochlorococcus marinus strain PCC 9511, a picoplanktonic cyanobacterium, synthesizes the smallest urease
Utility of polymerase chain reaction assays for confirmation of liquid culture test results for Ureaplasma spp.
Development of a selective and sensitive polymerase chain reaction assay for the detection of Mycoplasma pirum
Utility of Polymerase Chain Reaction Assays for Confirmation of Liquid Culture Test Results for Ureaplasma spp.
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