Ureolytic/Non-Ureolytic Bacteria Co-Cultured Self-Healing Agent for Cementitious Materials Crack Repair

Materials
Hyeong Min SonHaeng Ki Lee

Abstract

The present study investigated the CaCO₃ precipitation performance of ureolytic and non-ureolytic bacteria co-cultured as a self-healing agent for cementitious materials crack repair. Three different inoculum ratios of ureolytic Sporosarcina pasteurii and non-ureolytic Bacillus thuringiensis (10:0, 8:2, or 5:5) were used. The effect of coculturing ureolytic and non-ureolytic bacteria on microbial metabolism was investigated by measuring the rate of growth in urea-containing medium and the rate of NH₄⁺ and CaCO₃ production in urea⁻calcium lactate medium. The self-healing efficiency of co-cultured bacteria was examined by exposing cement mortar specimens with predefined cracks to media containing single urease-producing or co-cultured bacteria. The obtained results provide new findings, where CaCO₃ precipitation is improved by co-culturing ureolytic and non-ureolytic bacteria, owing to the relatively faster growth rate of non-ureolytic bacteria. The crack filling rate correlated with the width of crack, in particular, specimens with a smaller crack width showed the faster filling effect, indicating that the crack width can be a dominant factor influencing the CaCO₃ precipitation capacity of co-cultured bacteria.

References

Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·E SchnepfD H Dean
Mar 10, 2001·Enzyme and Microbial Technology·S S. BangV Ramakrishnan
May 2, 2009·Journal of Industrial Microbiology & Biotechnology·V AchalM Sudhakara Reddy
Sep 20, 2011·Journal of Industrial Microbiology & Biotechnology·J Y WangW Verstraete
Jul 20, 2016·Journal of Hazardous Materials·J G JangH K Lee

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Citations

Sep 7, 2020·Bioprocess and Biosystems Engineering·Romario P SantosRanyere L Souza

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