Ureteral Reconstruction in Goats Using Tissue-Engineered Templates and Subcutaneous Preimplantation

Tissue Engineering. Part a
Paul K J D de JongeWout F J Feitz

Abstract

Repair of long ureteral defects often requires long graft tissues and extensive surgery. This is associated with complications, including a lack of suitable tissue and graft site morbidity. Tissue engineering may provide an attractive alternative to the autologous graft tissues. In this study, ureteral repair using (preimplanted) tubular collagen-Vicryl templates was evaluated in a new goat model. Tubular templates were prepared from tubularized Vicryl meshes and 0.7% type-I collagen (length = 6 cm, inner diameter = 6 mm, wall thickness = 3 mm). In total, twelve goats were used and evaluated after 3 months. Eight goats were implanted with the collagen-Vicryl templates and in four goats the templates were first preimplanted in the subcutis and subsequently used as ureteral graft. Template implantation was successful in 92% of the goats(11/12). During follow-up, 82% of the animals (9/11) survived without signs of discomfort. Two animals were sacrificed prematurely due to kidney perforation by the stent and urine leakage. Two other animals presented with stenosis of the neoureter due to stent migration. After preimplantation, the templates were remodeled mostly to autologous tissue with similar mechanical characteristics as the na...Continue Reading

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Citations

Feb 23, 2019·Tissue Engineering. Part B, Reviews·Heinz P JankeEgbert Oosterwijk
Aug 11, 2020·BioMed Research International·Shengwei XiongDaniel D Eun
Oct 30, 2020·Frontiers in Bioengineering and Biotechnology·Masoud ZamaniMohammad Naji
Apr 4, 2021·Biology·Rui Damásio AlvitesAna Colette Maurício

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Methods Mentioned

BETA
hysterectomy
scanning electron microscopy
two hybrid
X-ray

Software Mentioned

SPSS

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