Urethral reconstruction using oral keratinocyte seeded bladder acellular matrix grafts

The Journal of Urology
Chao LiShuo Yin

Abstract

We investigated the feasibility of urethral reconstruction using oral keratinocyte seeded bladder acellular matrix grafts. Autologous oral keratinocytes were isolated, expanded and seeded onto bladder acellular matrix grafts to obtain a tissue engineered mucosa. The tissue engineered mucosa was assessed using morphology and scanning electron microscopy. In 24 male rabbits a ventral urethral mucosal defect was created. Urethroplasty was performed with autogenic oral keratinocyte seeded bladder acellular matrix grafts in 12 rabbits in the experimental group or with bladder acellular matrix grafts with no cell seeding in 12 in the control group. Retrograde urethrography was performed 1, 2 and 6 months after grafting. The urethral grafts were analyzed grossly and histologically. Oral keratinocytes had good biocompatibility with bladder acellular matrix grafts. Rabbits implanted with oral keratinocyte seeded bladder acellular matrix grafts voided without difficulty. Retrograde urethrography revealed no sign of strictures at 1, 2 and 6 months. In the control group the urethra with repaired defects was accompanied by strictures. Histological examination showed that grafts seeded with oral keratinocytes formed a 1-layer structure by 1 ...Continue Reading

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