Abstract
We conducted this study to evaluate the combined effect of acellular bladder submucosa matrix (BSM) and autologous urethral tissue for the treatment of long segment urethral stricture in a rabbit model. To prepare the BSM, porcine bladder submucosa was processed, decellularized, configured into a sheet-like shape, and sterilized. Twenty rabbits were randomized to normal control, urethral stricture, urethroplasty using BSM only or BSM/autologous urethral tissue (n=5 per group). Retrograde urethrography was performed at 4, 8, and 12 weeks postoperatively, and the grafted specimens were harvested at week 12 to evaluate urethral reconstruction through histopathologic and immunohistochemical analysis. The mean urethral width of the control, stricture, BSM, and BSM/autologous urethral tissue groups at week 12 was 10.3±0.80, 3.8±1.35, 8.8±0.84, and 9.1±1.14 mm, respectively. The histopathologic study revealed that the BSM/autologous urethral tissue graft had a normal area of urethral lumen, compact muscular layers, complete epithelialization, and progressive infiltration by vessels in the regenerated urethra. In contrast, the BSM grafts revealed keratinized epithelium, abundant collagenized fibrous connective tissue, and were devoid o...Continue Reading
References
Aug 1, 1992·The Journal of Urology·J L FairbanksK E Bove
Aug 1, 1995·The Journal of Urology·S N BüyükünalS Dervişoğlu
Feb 1, 1996·Plastic and Reconstructive Surgery·W A MorrisonS Kumta
Aug 1, 1996·The Journal of Urology·R S SutherlandG R Cunha
Aug 1, 1996·The Journal of Urology·B P KroppK B Thor
Apr 1, 1997·British Journal of Urology·M ProbstE A Tanagho
Dec 31, 1997·Urology·S E DahmsE A Tanagho
Mar 12, 1998·Urology·J J YooA Atala
Jul 22, 1998·Urology·B P KroppK B Thor
Jan 12, 1999·British Journal of Urology·W SabbaghR A Brown
Aug 24, 1999·The Journal of Urology·A AtalaA B Retik
May 3, 2000·BJU International·P A MerguerianA E Khoury
Oct 10, 2002·The Urologic Clinics of North America·Alan B Retik, Anthony Atala
Oct 24, 2003·European Urology·Franco MantovaniEnrico Pisani
Jun 1, 2005·The Journal of Surgical Research·Stephen F BadylakAlejandro Nieponice
Oct 18, 2005·BJU International·Yuanyuan ZhangBradley P Kropp
Feb 20, 2007·European Urology·Richard FialaVladimir Student
Jul 10, 2007·Biomaterials·So Young ChunJames J Yoo
Jan 30, 2008·Journal of Biomedical Materials Research. Part a·Donald O FreytesStephen F Badylak
Dec 17, 2009·Plastic and Reconstructive Surgery·Lee L Q Pu
Nov 3, 2010·Plastic and Reconstructive Surgery·Jason P GlotzbachGeoffrey C Gurtner
Jan 24, 2012·The Journal of Urology·Karl-Dietrich SievertJ Seibold
May 1, 2012·World Journal of Urology·George VaosConstantinos Simopoulos
Aug 1, 1996·Pediatric Surgery International·C LorenzK L Wang
Nov 2, 2013·Urology·Bum Soo KimTae Gyun Kwon
Dec 24, 2013·Korean journal of urology·Hyun Su JungBum Soo Kim
Citations
Feb 4, 2016·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Na QiHong Tian
Sep 22, 2016·SpringerPlus·Jia LiYujie Wang
Sep 13, 2017·Experimental Biology and Medicine·Stanislav ŽiaranL'uboš Danišovič
Jan 19, 2019·Journal of Tissue Engineering and Regenerative Medicine·Jan AdamowiczTomasz Drewa
May 12, 2018·Molecular Medicine Reports·Binqiang TianYonghui Li
Aug 16, 2019·Tissue Engineering and Regenerative Medicine·Zahra RashidbenamMin Hwei Ng
Apr 13, 2019·International Journal of Molecular Sciences·Tariq O AbbasCristian P Pennisi
Jun 14, 2019·World Journal of Urology·Christopher Chapple
Feb 7, 2020·Nature Reviews. Urology·Yvonne Y ChanArun K Sharma
Apr 4, 2021·International Journal of Molecular Sciences·Zahra RashidbenamMin Hwei Ng
Jun 6, 2020·Biomedical Materials·Igor VasyutinYuanyuan Zhang
Aug 7, 2021·Bioengineering·Christophe CaneparoStéphane Bolduc
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Tariq O AbbasCristian Pablo Pennisi