Urethroplasty using autologous urethral tissue-embedded acellular porcine bladder submucosa matrix grafts for the management of long-segment urethral stricture in a rabbit model

Journal of Korean Medical Science
So Young ChunHyun Tae Kim

Abstract

We conducted this study to evaluate the combined effect of acellular bladder submucosa matrix (BSM) and autologous urethral tissue for the treatment of long segment urethral stricture in a rabbit model. To prepare the BSM, porcine bladder submucosa was processed, decellularized, configured into a sheet-like shape, and sterilized. Twenty rabbits were randomized to normal control, urethral stricture, urethroplasty using BSM only or BSM/autologous urethral tissue (n=5 per group). Retrograde urethrography was performed at 4, 8, and 12 weeks postoperatively, and the grafted specimens were harvested at week 12 to evaluate urethral reconstruction through histopathologic and immunohistochemical analysis. The mean urethral width of the control, stricture, BSM, and BSM/autologous urethral tissue groups at week 12 was 10.3±0.80, 3.8±1.35, 8.8±0.84, and 9.1±1.14 mm, respectively. The histopathologic study revealed that the BSM/autologous urethral tissue graft had a normal area of urethral lumen, compact muscular layers, complete epithelialization, and progressive infiltration by vessels in the regenerated urethra. In contrast, the BSM grafts revealed keratinized epithelium, abundant collagenized fibrous connective tissue, and were devoid o...Continue Reading

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Citations

Feb 4, 2016·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Na QiHong Tian
Sep 13, 2017·Experimental Biology and Medicine·Stanislav ŽiaranL'uboš Danišovič
Jan 19, 2019·Journal of Tissue Engineering and Regenerative Medicine·Jan AdamowiczTomasz Drewa
May 12, 2018·Molecular Medicine Reports·Binqiang TianYonghui Li
Aug 16, 2019·Tissue Engineering and Regenerative Medicine·Zahra RashidbenamMin Hwei Ng
Apr 13, 2019·International Journal of Molecular Sciences·Tariq O AbbasCristian P Pennisi
Jun 14, 2019·World Journal of Urology·Christopher Chapple
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Jun 6, 2020·Biomedical Materials·Igor VasyutinYuanyuan Zhang
Aug 7, 2021·Bioengineering·Christophe CaneparoStéphane Bolduc
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Tariq O AbbasCristian Pablo Pennisi

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Methods Mentioned

BETA
biopsy

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