PMID: 8595979Aug 1, 1995Paper

URF13, a ligand-gated, pore-forming receptor for T-toxin in the inner membrane of cms-T mitochondria

Journal of Bioenergetics and Biomembranes
D M RhoadsJ N Siedow

Abstract

URF13 is the product of a mitochondrial-encoded gene (T-urf13) found only in maize plants containing the Texas male-sterile cytoplasm (cms-T), and it is thought to be responsible for both cytoplasmic male sterility and the susceptibility of cms-T maize to the fungal pathogens Bipolaris maydis race T and Phyllosticata maydis. Mitochondria isolated from cms-T maize are uniquely sensitive to pathotoxins (T-toxin) produced by these fungi and to methomyl (a commercial insecticide). URF13 acts as a receptor that specifically binds T-toxin to produce hydrophilic pores in the inner mitochondrial membrane. When expressed in Escherichia coli cells, URF13 also forms hydrophilic pores in the plasma membrane if exposed to T-toxin or methomyl. Topological studies established that URF13 contains three membrane-spanning alpha-helices, two of which are amphipathic and can contribute to pore formation. Chemical cross-linking of URF13 was used to demonstrate the existence of URF13 oligomers in cms-T mitochondria and E. coli cells. The ability of the carboxylate-specific reagent, N,N'-dicyclohexycarbodiimide, to cross-link URF13 was used in conjunction with site-directed mutagenesis to establish that the URF13 tetramer has a central core consistin...Continue Reading

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Citations

Mar 16, 2004·The Journal of Biological Chemistry·Evgeny V KuzminKathleen J Newton
Dec 10, 2013·Annual Review of Plant Biology·Letian Chen, Yao-Guang Liu
Jun 25, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Reetu TutejaRajeev K Varshney
May 1, 2001·The Science of the Total Environment·T SuramanaP Sinhaseni
Nov 9, 2005·Mitochondrion·Bettina Linke, Thomas Börner
Sep 10, 2019·ACS Chemical Biology·Brendan J BattersbyOmid Safronov

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