URF6, last unidentified reading frame of human mtDNA, codes for an NADH dehydrogenase subunit

Science
A ChomynG Attardi

Abstract

The polypeptide encoded in URF6, the last unassigned reading frame of human mitochondrial DNA, has been identified with antibodies to peptides predicted from the DNA sequence. Antibodies prepared against highly purified respiratory chain NADH dehydrogenase from beef heart or against the cytoplasmically synthesized 49-kilodalton iron-sulfur subunit isolated from this enzyme complex, when added to a deoxycholate or a Triton X-100 mitochondrial lysate of HeLa cells, specifically precipitated the URF6 product together with the six other URF products previously identified as subunits of NADH dehydrogenase. These results strongly point to the URF6 product as being another subunit of this enzyme complex. Thus, almost 60% of the protein coding capacity of mammalian mitochondrial DNA is utilized for the assembly of the first enzyme complex of the respiratory chain. The absence of such information in yeast mitochondrial DNA dramatizes the variability in gene content of different mitochondrial genomes.

Citations

Dec 1, 1989·Annals of Neurology·W D ParkerJ K Parks
Dec 18, 2001·Prenatal Diagnosis·L E NiersL P van den Heuvel
Nov 1, 1990·Journal of Neurology·L BetP Cerretelli
Apr 1, 1988·Journal of Bioenergetics and Biomembranes·H R Scholte
Oct 1, 1991·Journal of Bioenergetics and Biomembranes·H Weiss, T Friedrich
May 1, 1994·Molecular Biology Reports·H J Pel, L A Grivell
Nov 1, 1996·Somatic Cell and Molecular Genetics·Z A SherifC D Whitfield-Broome
Feb 1, 1996·Molecular Neurobiology·F BlandiniJ T Greenamyre
Dec 2, 2010·Journal of Bioenergetics and Biomembranes·Bilal Amarneh, Steven B Vik
Oct 1, 1989·Developmental Biology·A A AlcivarN B Hecht
Jan 1, 1990·Free Radical Biology & Medicine·B Bandy, A J Davison
May 9, 2002·Biochimica Et Biophysica Acta·Pamela DavidMoshe Finel
Jun 19, 1998·Biochimica Et Biophysica Acta·T YagiA Matsuno-Yagi
Dec 30, 1988·Biochemical and Biophysical Research Communications·V J Clay, C I Ragan

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