Uric acid quantification in fingernail of gout patients and healthy volunteers using HPLC-UV

Biomedical Chromatography : BMC
Xi-Ling LiJun Zhe Min

Abstract

The presence of elevated uric acid (UA) levels is a sign of gout, that is, hyperuricemia. In this study the monitoring of the UA levels in less-invasive biological samples, such as the human fingernail, is suggested for the diagnosis and therapy of gout. Twenty-six healthy volunteers (HV) and 22 gout patients (GP) were studied. The UA was extracted from human fingernail samples, then separated on an Inertsil ODS-3 column (250 × 4.6 mm i.d., 4.0 μm, GL Sciences) by isocratic elution using methanol-74 mm phosphate buffer (pH 2.2) 2:98 (v/v). A UV detector was used to monitor the samples at 284 nm. Using the developed method, different UA concentrations were found in the GP and HV. When comparing the concentrations from GP with those from HV, a statistically significant correlation was observed between the UA (p < 0.01). In this study, the UA was confirmed as a potential biomarker for the diagnosis and therapy of gout. We have developed a novel sensitive, and simple method for the determination of UA in the fingernails of GP and HV. The human fingernail may serve as a noninvasive biosample for the diagnosis of gout. Copyright © 2016 John Wiley & Sons, Ltd.

References

Sep 27, 1969·British Medical Journal·G A Rail
Feb 23, 2002·Joint, Bone, Spine : Revue Du Rhumatisme·Vincent GuggiJean-Charles Gerster
Jan 22, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Koichi InoueHiroyuki Nakazawa
Apr 25, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Nancy CooperJean W Lee
Aug 21, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xinhua DaiBei Xu
Mar 3, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xingnan Li, Adrian A Franke
Mar 1, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun Zhe MinToshimasa Toyo'oka
Mar 25, 2011·The American Journal of Dermatopathology·Mariantonieta Tirado-González, Aldo González-Serva
Aug 27, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yun LiuXiaofang Yang
May 3, 2013·Current Medical Research and Opinion·L Punzi, A So
Oct 15, 2013·Biomedical Chromatography : BMC·Jun Zhe MinToshimasa Toyo'oka
Apr 11, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nidhi ChauhanC S Pundir
Sep 14, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xi-Ling LiJun Zhe Min

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Citations

Aug 7, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Qingkun MaJun Zhe Min
Sep 25, 2020·Scientific Reports·Haibing ChenJun Zhe Min
Nov 3, 2021·Critical Reviews in Clinical Laboratory Sciences·Sarahi Jaramillo OrtizFrédéric J Tessier

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