Uricase grafted nanoconducting matrix based electrochemical biosensor for ultrafast uric acid detection in human serum samples

International Journal of Biological Macromolecules
Shilpi VermaSurinder P Singh

Abstract

Gold nanoparticles decorated graphene oxide (Au-rGO) nanocomposite thin films with enhanced electro-active characteristics were prepared and covalently immobilized with uricase (UOx) enzyme for sensitive and selective detection of uric acid (UA). Differential pulse voltammetry (DPV) studies revealed rapid response of fabricated electrode towards UA at low potential (0.228 V) in a wide concentration range of 50-800 μM with a sensitivity of 86.62 ± 0.19 μA mM-1 and very low detection limit of 7.32 ± 0.21 μM. The obtained Michaelis-Menten constant (km) value of 51.75 μM signifies high enzyme kinetics at electrode surface with UA. The developed biosensor was successfully applied to detect UA in human serum samples. Interferences due to components present in the real matrix were evaluated and UA determination in mixed sample was also performed. The fabricated UOx/Au-rGO/ITO biosensor demonstrated high reproducibility and a shelf-life of 6 months indicating the promising future of Au-rGO nanocomposite as an efficient transducer matrix for biosensing applications. The fast response time (1.0 ± 0.6 s) and improved sensor performance is attributed to the synergistic electronic properties of Au-nanoparticles and rGO that provided enhance...Continue Reading

Citations

Aug 28, 2020·Frontiers in Chemistry·Congcong Zhang, Xin Du
Sep 29, 2019·Critical Reviews in Analytical Chemistry·Masoumeh GhalkhaniSibel A Ozkan
Sep 22, 2020·Critical Reviews in Analytical Chemistry·Nurgul K BakirhanSibel A Ozkan
Dec 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tamanna IslamA J Saleh Ahammad
Oct 15, 2020·Chemical Reviews·Hui ChenShelley D Minteer
Nov 9, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Xing LuFengyu Qu
Dec 28, 2021·Biotechnology and Bioengineering·Aditi SammiPranjal Chandra

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