PMID: 6983457Nov 1, 1982Paper

Uridine as the only alternative to pyrimidine de novo synthesis in rat T lymphocytes

FEBS Letters
G BismuthP H Cartier

Abstract

Concanavalin A-induced proliferation of rat T-lymphocytes is completely inhibited by 10(-5) M pyrazofurin, a potent inhibitor of pyrimidine de novo synthesis, as judged by cell viability and [3H]thymidine incorporation. Proliferation is completely restored by 5 X 10(-5) M uridine. Cytidine, deoxycytidine, deoxyuridine and thymidine 10 X 10(-5) M each, fail to re-establish proliferation but produce an isotropic dilution of [3H]thymidine uptake in DNA. Bases (cytosine, uracil and thymine) neither restore proliferation nor induce isotopic dilution. The unexpected inability of cytidine to reverse de novo pyrimidine synthesis inhibition suggests a lack of cytidine deaminase activity in rat T-lymphocytes. This is confirmed by a direct sensitive radioisotopic assay (less than 0.001 nmol X min-1 X 10(-6) cells).

References

May 1, 1975·The Biochemical Journal·S D Barlow, M G Ord
Jun 1, 1968·British Journal of Haematology·J MetzV Herbert
Oct 1, 1981·European Journal of Immunology·L ThuillierP H Cartier

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Citations

Nov 9, 1994·Molecular and Cellular Biochemistry·T W Traut
Apr 15, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·J L PérignonP H Cartier
Jan 1, 1986·International Journal of Immunopharmacology·J L PérignonP H Cartier
Jan 1, 2015·Cell Death Discovery·N DasguptaR Li
Nov 4, 1998·Archives of Biochemistry and Biophysics·P A Ropp, T W Traut

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