Uridylylation of the potyvirus VPg by viral replicase NIb correlates with the nucleotide binding capacity of VPg.

The Journal of Biological Chemistry
Pietri Puustinen, Kristiina Mäkinen

Abstract

Poty- and picornaviruses share similar genome organizations and polyprotein processing strategies. By analogy to picornaviruses it has been proposed that the genome-linked protein VPg may serve as a primer for genome replication of potyviruses. The multifunctional VPg of potato virus A (PVA; genus Potyvirus) was found to be uridylylated by NIb, the RNA polymerase of PVA. The nucleotidylation activity of NIb is more efficient in the presence of Mn(2+) than Mg(2+) and does not require an RNA template. Our results suggest that the nucleotidylation reaction exhibits weak preference for UTP over the other NTPs. An NTP-binding experiment with oxidized [alpha-(32)P]UTP revealed that PVA VPg contains an NTP-binding site. Deletion of a 7-amino acid-long putative NTP-binding site from VPg reduced nucleotide-binding capacity and debilitated uridylylation reaction. These results provide evidence that VPg may play a similar role in RNA synthesis of potyviruses as it does in the case of picornaviruses.

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P G RothbergE Wimmer
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·N M Crawford, D Baltimore
May 16, 1995·Biochemistry·O C RichardsE Ehrenfeld
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·X H Li, J C Carrington
Apr 1, 1994·Journal of Virology·M A Restrepo-Hartwig, J C Carrington
Sep 12, 1997·The Journal of Biological Chemistry·O C Richards, E Ehrenfeld
Aug 31, 2000·Journal of Molecular Biology·C NotredameJ Heringa
Jan 21, 2001·The Journal of Biological Chemistry·K I IvanovK Mäkinen
Mar 10, 2001·Nature·S J ButcherD I Stuart
Oct 30, 2001·Advances in Virus Research·E SadowyA L Haenni
Nov 20, 2002·Journal of Virology·Pietri PuustinenKristiina Mäkinen
Apr 15, 2003·Journal of Virology·Shane CrottyRaul Andino

❮ Previous
Next ❯

Citations

Jun 24, 2011·Journal of Virology·Katri EskelinKristiina Mäkinen
Dec 24, 2010·Journal of Virology·Kimmo I RantalainenKristiina Mäkinen
Nov 18, 2009·Biochemistry. Biokhimii︠a︡·Chaojiang GuCongyi Zheng
Dec 4, 2012·Viruses·Jiri SochorRene Kizek
Jan 11, 2014·Molecular Plant-microbe Interactions : MPMI·M SorelS German-Retana
Jun 7, 2014·Annual Review of Phytopathology·Pooja Saxena, George P Lomonossoff
Feb 3, 2016·BMC Genomics·Fernando MartínezJosé-Antonio Daròs
Sep 20, 2012·Biochemical and Biophysical Research Communications·Chhavi Mathur, Handanahal S Savithri
Mar 24, 2012·Current Opinion in Virology·Jun Jiang, Jean-François Laliberté
Oct 10, 2013·Molecular Plant Pathology·Juan Antonio GarcíaThierry Candresse
Mar 17, 2006·Peptides·Catherine H ScheinWerner Braun
Dec 14, 2005·Trends in Plant Science·Christophe Robaglia, Carole Caranta
Sep 13, 2015·Biochemical and Biophysical Research Communications·Yushan ZhaiJingsheng Xu
Apr 12, 2014·The Journal of General Virology·K I IvanovK Mäkinen
Oct 6, 2009·Virology·Kimmo I RantalainenKristiina Mäkinen
Apr 12, 2014·Frontiers in Plant Science·Kristiina Mäkinen, Anders Hafrén
Nov 18, 2015·Molecular Plant Pathology·Andres LõhmusKristiina Mäkinen
Nov 1, 2007·The Journal of Biological Chemistry·Renata GrzelaJadwiga Chroboczek
Nov 26, 2015·International Journal of Biological Macromolecules·R B MariuttiR K Arni
Aug 14, 2010·The Journal of Organic Chemistry·Gerbrand J van der Heden van NoortDmitri V Filippov

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.