Urinalysis of 4-hydroxynonenal, a marker of oxidative stress, using stir bar sorptive extraction-thermal desorption-gas chromatography/mass spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Adriana StopforthPat Sandra

Abstract

A simple and fast method for the measurement of 4-hydroxynonenal (4HNE), a highly toxic end-product of lipid peroxidation, in urine samples is described. The method combines stir bar sorptive extraction (SBSE) with two derivatization steps, followed by thermal desorption and GC/MS. 4HNE is derivatized in situ with O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine and the oxime is extracted from the aqueous phase with SBSE. The 4HNE-oxime is further acylated by headspace derivatization prior to thermal desorption. Derivatization reactions and extraction were optimized in terms of reagent quantities, temperature and time. The method is linear over a concentration range of 0.5-5 ng mL(-1) with a correlation coefficient of 0.997. The limit of detection and limit of quantitation are 22 and 75 pg mL(-1) urine, respectively. The high sensitivity of the method allows the measurement of physiological concentrations of 4HNE in urine samples.

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Citations

Sep 4, 2009·Analytical and Bioanalytical Chemistry·Hiroyuki Kataoka
Oct 19, 2013·Analytical and Bioanalytical Chemistry·Man HeBin Hu
Sep 15, 2010·Free Radical Research·Corinne M SpickettNeven Zarkovic
Jan 17, 2012·Free Radical Biology & Medicine·Qingling LiGuo-Fang Zhang
Jun 29, 2011·Free Radical Biology & Medicine·Piotr Zimniak
Jan 8, 2008·Journal of Chromatography. a·Erwin Kaal, Hans-Gerd Janssen
Jan 24, 2007·Journal of Chromatography. a·Frank David, Pat Sandra
Mar 12, 2009·Journal of Separation Science·Fernando M LancasIgor R B Olivares
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Dec 7, 2007·Medicinal Research Reviews·G PoliG Leonarduzzi
Dec 17, 2020·Comprehensive Reviews in Food Science and Food Safety·Xuxia ZhouYongning Wu

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