Urinary beta2-microglobulin is associated with acute renal allograft rejection

American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation
William S OettingHassan N Ibrahim

Abstract

Identifying urinary biomarkers associated with acute rejection (AR) of kidney allografts could improve recipient care by allowing AR to be diagnosed noninvasively and treated earlier. We attempted to identify novel biomarkers associated with AR by analyzing urinary proteins by using matrix-associated laser desorption ionization time-of-flight mass spectroscopy (MALDI-TOF MS). Using MALDI-TOF MS, we analyzed urine samples from 30 renal allograft recipients with biopsy-proven AR, 15 allograft recipients without AR, preoperative samples from 29 kidney donors, and 10 subjects with proteinuric native kidney disease. In samples obtained at the time of AR, we identified a protein peak at 11.7 kd that correlated strongly with AR. In regard to its predictive power for AR, this protein peak showed sensitivity of 83.3%, specificity of 80%, positive predictive value of 89%, and negative predictive value of 70.6%, suggesting that this protein is highly associated with AR. We identified this peak as being beta2-microglobulin. This was validated by using enzyme-linked immunosorbent assay, which documented the presence of high urinary beta2-microglobulin levels in subjects with AR. Beta2-microglobulin could be a strong biomarker for AR if used...Continue Reading

References

Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Dec 9, 2000·Transplant International : Official Journal of the European Society for Organ Transplantation·J Kaden, R Priesterjahn
May 2, 2003·Annals of Surgery·William ClarkeErnesto P Molmenti
Oct 9, 2003·Kidney International·Joris J T H RoelofsSandrine Florquin
Oct 28, 2003·Transplantation·Mahmoud SadeghiGerhard Opelz
Dec 19, 2003·Advances in Renal Replacement Therapy·James F WinchesterNathan W Levin
Dec 25, 2003·Journal of the American Society of Nephrology : JASN·William L Whittier, Stephen M Korbet
Dec 25, 2003·Journal of the American Society of Nephrology : JASN·Stefan SchaubPeter Nickerson
Jun 16, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Sundaram HariharanHamid Rabb
Dec 24, 2004·Current Opinion in Critical Care·Won K Han, Joseph V Bonventre
Mar 12, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Stefan SchaubPeter Nickerson

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Citations

Jan 3, 2013·EMBO Molecular Medicine·Alex KentsisSusan Kim
Nov 18, 2008·Journal of Proteome Research·Sanjeev K AkkinaHassan N Ibrahlm
Jun 23, 2009·Transplantation·Yan ZhangGary L Nelsestuen
Feb 28, 2007·Pharmacogenomics·Glen L Hortin, Denis Sviridov
Jun 26, 2009·QJM : Monthly Journal of the Association of Physicians·K BramhamA J Thompson
Oct 25, 2011·Néphrologie & thérapeutique·Nada Alachkar
Apr 3, 2007·Neoplasia : an International Journal for Oncology Research·Niko EscherFerdinand von Eggeling
Nov 21, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Toshimitsu Niwa
Nov 28, 2007·International Immunopharmacology·Sunil KurianDaniel R Salomon
Sep 1, 2009·Proteomics. Clinical Applications·Alex KentsisHanno Steen
Sep 4, 2008·Pediatric Transplantation·Tara K Sigdel, Minnie M Sarwal
Apr 30, 2014·Veterinary Clinical Pathology·Laura E BrandtJessica E Prenni
Mar 5, 2015·Proteomics. Clinical Applications·Judy GopalHui-Fen Wu
May 23, 2014·The Korean Journal of Internal Medicine·Jae Ryung ShinChun Gyoo Ihm
Jun 4, 2011·Journal of Proteomics·Ivana PešićHassan Dihazi
Feb 19, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Na GaoKewu Huang
Dec 12, 2018·Therapeutic Drug Monitoring·Benjamin R GriffinCharles L Edelstein
May 20, 2020·Journal of Clinical Medicine·Charat ThongprayoonWisit Cheungpasitporn
Sep 24, 2020·International Journal of Molecular Sciences·Francesco GuzziPaola Romagnani
Jul 1, 2017·Frontiers in Medicine·Christos P ArgyropoulosAntonios H Tzamaloukas
Mar 19, 2019·Therapeutic Drug Monitoring·Benjamin R GriffinCharles L Edelstein
Feb 23, 2013·Annals of Clinical Biochemistry·Maarten G J SnoeijsWill K W H Wodzig

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