Urinary candidate biomarker discovery in a rat unilateral ureteral obstruction model

Scientific Reports
Yuan YuanYouhe Gao

Abstract

Urine has the potential to become a better source of biomarkers. Urinary proteins are affected by many factors; therefore, differentiating between the variables associated with any particular pathophysiological condition in clinical samples is challenging. To circumvent these problems, simpler systems, such as animal models, should be used to establish a direct relationship between disease progression and urine changes. In this study, a unilateral ureteral obstruction (UUO) model was used to observe tubular injury and the eventual development of renal fibrosis, as well as to identify differential urinary proteins in this process. Urine samples were collected from the residuary ureter linked to the kidney at 1 and 3 weeks after UUO. Five hundred proteins were identified and quantified by LC-MS/MS, out of which 7 and 19 significantly changed in the UUO 1- and 3-week groups, respectively, compared with the sham-operation group. Validation by western blot showed increased levels of Alpha-actinin-1 and Moesin in the UUO 1-week group, indicating that they may serve as candidate biomarkers of renal tubular injury, and significantly increased levels of Vimentin, Annexin A1 and Clusterin in the UUO 3-week group, indicating that they may...Continue Reading

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Citations

Jan 6, 2016·Journal of Clinical Medicine·Anusha H TennakoonJyoji Yamate
Jan 5, 2016·Scientific Reports·Chao-Yung WangMing-Shien Wen
Jun 9, 2016·Clinical Kidney Journal·Theofilos PapadopoulosJulie Klein
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Jun 2, 2021·Expert Review of Proteomics·Jing Wei, Youhe Gao
Jan 14, 2017·The American Journal of Pathology·Mark PinesOrna Halevy

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Methods Mentioned

BETA
proteomic profiling
urine collected
deamidation

Software Mentioned

Scaffold
Mascot
Statistical Package for Social Studies ( SPSS )
Proteome
ImageJ

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