Urinary Cell-Free DNA Quantification as Non-Invasive Biomarker in Patients with Bladder Cancer

Urologia Internationalis
Antonin BrisudaMarek Babjuk

Abstract

Concentration of urinary cell-free DNA (ucfDNA) belongs to potential bladder cancer markers, but the reported results are inconsistent due to the use of various non-standardised methodologies. The aim of the study was to standardise the methodology for ucfDNA quantification as a potential non-invasive tumour biomarker. In total, 66 patients and 34 controls were enrolled into the study. Volumes of each urine portion (V) were recorded and ucfDNA concentrations (c) were measured using real-time PCR. Total amounts (TA) of ucfDNA were calculated and compared between patients and controls. Diagnostic accuracy of the TA of ucfDNA was determined. The calculation of TA of ucfDNA in the second urine portion was the most appropriate approach to ucfDNA quantification, as there was logarithmic dependence between the volume and the concentration of a urine portion (p = 0.0001). Using this methodology, we were able to discriminate between bladder cancer patients and subjects without bladder tumours (p = 0.0002) with area under the ROC curve of 0.725. Positive and negative predictive value of the test was 90 and 45%, respectively. Quantification of ucf DNA according to our modified method could provide a potential non-invasive biomarker for di...Continue Reading

Citations

Sep 4, 2018·Journal of Clinical Laboratory Analysis·Pei LiYu Wang
Dec 5, 2019·Expert Review of Molecular Diagnostics·Antonio Lopez-BeltranRodolfo Montironi
Sep 25, 2018·Frontiers in Oncology·Giorgio SantoniNicola Battelli
Apr 16, 2017·Molecular Cancer·Ashley Di MeoGeorge M Yousef
Apr 4, 2019·Cytopathology : Official Journal of the British Society for Clinical Cytology·Antonín BrisudaMarek Babjuk
Nov 20, 2020·International Journal of Molecular Sciences·Zuzana PösTomas Szemes
Mar 7, 2021·Diagnostics·Ryan Tsz-Hei TseJeremy Yuen-Chun Teoh
Sep 1, 2021·Critical Reviews in Oncology/hematology·Sasimol UdomrukParunya Chaiyawat

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