Urinary connective tissue growth factor increases far earlier than histopathological damage and functional deterioration in early chronic renal allograft injury

Scandinavian Journal of Urology and Nephrology
Yujun ShiHong Bu

Abstract

To date, serum biochemistry examination and routine biopsy are the most commonly used methods to assess renal function after allogenic kidney transplantation. Connective tissue growth factor (CTGF) has been considered as a biomarker of chronic renal allograft injury characterized by tubular atrophy and interstitial fibrosis (TA/IF). This study explored the potential value of urinary CTGF as an early predictor of TA/IF using a rat model. A Fisher to Lewis allogenic rat kidney transplant model was established and the recipients were killed at weeks 4, 8 and 12 post-transplantation. TA/IF was graded based on Banff Schema 1997. The location and expression of CTGF mRNA were detected by oligonucleotide-primed in situ DNA synthesis and quantitative polymerase chain reaction. CTGF protein expression was examined with immunohistochemistry and immunoblotting. Urinary CTGF concentration was measured by enzyme-linked immunosorbent assay. The correlation between urinary CTGF concentration and serum creatinine (SCr) and Banff score was analysed statistically. Typical morphological changes including TA/IF in allograft appeared at week 8 and became very severe at week 12 post-transplantation. CTGF expression in epithelium was up-regulated earl...Continue Reading

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Jun 28, 2013·Transplantation·Christoph MetalidisDirk R J Kuypers
Sep 4, 2012·Cell Communication and Signaling : CCS·Jonathan ZuehlkeMargarete Goppelt-Struebe
Aug 9, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Péter Hamar, Dontscho Kerjaschki
Jun 17, 2016·American Journal of Physiology. Renal Physiology·María Del Pilar Sosa PeñaJ Andrés Melendez
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