Urinary excretion measurement of cysteine and homocysteine in the form of their S-pyridinium derivatives by high-performance liquid chromatography with ultraviolet detection

Journal of Chromatography. a
E KaniowskaE Bald

Abstract

Several human diseases, in particular metabolic disorders, often lead to the accumulation of characteristic metabolites in plasma, urine and cells. The selected diseases of this type include cystinuria and homocystinuria. In the typical laboratory diagnosis of these two diseases, a positive nitroprusside test is followed by quantitative analysis of urine cysteine and homocysteine in order to differentiate between cystinuria and homocystinuria. A sensitive and reproducible assay for total urine cysteine and homocysteine has been developed. The essential steps in the assay include conversion of disulphides to free thiols with tributylphosphine, conjugation of the thiols with 2-chloro-1-methyl pyridinium iodide, separation of S-pyridinium derivatives of cysteine and homocysteine from other endogenous urine thiol derivatives by reversed-phase high-performance liquid chromatography, and detection and quantitation by spectrophotometry. The method has a sensitivity of 4 pmol and is reproducible, intra- and inter-day coefficients of variation are from 1.37 to 4.14% and from 2.38 to 5.01%, respectively. The mean concentration of total urine cysteine and homocysteine in healthy donors (7 men and 7 women) were for women. 92.0 +/- 45.8 and...Continue Reading

References

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Citations

Jun 23, 2006·Analytical and Bioanalytical Chemistry·K KuśmierekE Bald
Feb 23, 2012·Journal of Biomedical Optics·Hongliang Tan, Yang Chen
Dec 4, 2012·Sensors·Hanjing PengBinghe Wang
Mar 4, 2014·Materials Science & Engineering. C, Materials for Biological Applications·K K AswiniV M Biju
Dec 21, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Behzad RezaeiSiavash Nouroozi
Nov 30, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·K KuśmierekE Bald
Apr 24, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Krzysztof KuśmierekEdward Bald
Jul 25, 2014·Archives of Biochemistry and Biophysics·Micaela PivatoAntonio Masi
Dec 10, 2014·Physical Chemistry Chemical Physics : PCCP·Jayasmita JanaTarasankar Pal
Nov 1, 2016·Journal of Fluorescence·N V KurnosovV A Karachevtsev
Oct 29, 2013·The Journal of Organic Chemistry·Ruixue PengXiaoming Feng
Jun 6, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Haibing Li, Xiaoqiong Wang
May 27, 2010·Chemical Society Reviews·Xiaoqiang ChenJuyoung Yoon
Jun 28, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Apostolia TsiasiotiParaskevas D Tzanavaras

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