Urinary excretion of protectin (CD59), complement SC5b-9 and cytokines in membranous glomerulonephritis

Kidney International
T LehtoS Meri

Abstract

Protectin (CD59) is a low molecular weight glycophosphoinositol-anchored inhibitor of the membrane attack complex of complement (MAC) that is present, for example, on the membranes of endothelial cells and on epithelial cells of glomeruli and distal tubuli. To examine for the possibility that CD59 becomes detached from cell surfaces following cell injury, this study evaluated renal excretion of CD59 in patients with idiopathic membranous glomerulonephritis (MGN; N = 21), diabetic nephropathy (DNP; N = 15) and in healthy control subjects (N = 13). CD59 in human urine was quantitated by a competitive solid-phase radioimmunoassay having approximately 13 kDa soluble urinary CD59 as a standard. Immunofluorescence microscopy demonstrated a decreased expression of CD59 in the glomeruli of MGN patients. Using a Triton X-114 phase separation method 91 to 97% of urinary CD59 was found to be in a soluble form without anchor-associated phospholipid. The mean (+/- SEM) level of urinary CD59 was 5.6 +/- 0.2 micrograms/ml in MGN patients, 3.7 +/- 0.4 micrograms/ml in healthy controls (P < 0.001) and 2.6 +/- 0.1 in DNP patients (P < 0.001). When related to urinary creatinine (UCr) the corresponding values were 11.9 +/- 5.6, 4.8 +/- 0.3 (P = 0....Continue Reading

References

Apr 1, 1992·Kidney International·P E BrenchleyN P Mallick
Dec 1, 1991·Kidney International·J L OgrodowskiW Kolb
Sep 11, 1991·Kidney International·M SchulzeO Gotze
Jul 1, 1989·Kidney International·C J PruchnoW G Couser
Jan 1, 1988·International Archives of Allergy and Applied Immunology·G M HänschB Sterzel
Mar 1, 1974·Journal of Clinical Pathology·G V Heimer, C E Taylor
Dec 1, 1993·Protein Science : a Publication of the Protein Society·C M FletcherD Neuhaus
Oct 1, 1993·European Journal of Immunology·S MeriR Renkonen

❮ Previous
Next ❯

Citations

Dec 1, 1995·Immunotechnology : an International Journal of Immunological Engineering·Y Sugita, Y Masuho
Jul 27, 2001·Clinical and Experimental Immunology·M Närkiö-Mäkelä, S Meri
Dec 9, 1998·Kidney International·M Nangaku
Jun 2, 1998·Kidney International·B J Ballermann
May 7, 1999·Kidney International·M Kitamura, L G Fine
Oct 9, 1998·The Journal of Experimental Medicine·B SchillerR J Quigg
Dec 12, 2013·Pathology Research International·Miroslav Sekulic, Simona Pichler Sekulic
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·J AcostaJ Halperin
Feb 13, 2008·Scandinavian Journal of Gastroenterology·Outi LindströmLeena Kylänpää
Dec 19, 2014·American Journal of Reproductive Immunology : AJRI·Stephanie H GusehRichard M Burwick
Nov 27, 2007·Expert Opinion on Pharmacotherapy·Piero RuggenentiGiuseppe Remuzzi
Apr 26, 2000·Clinical Immunology : the Official Journal of the Clinical Immunology Society·T ShojiT Seya
Dec 31, 2004·Journal of the American Society of Nephrology : JASN·Masanori HaraTadahiro Kajita
Feb 10, 2018·Multiple Sclerosis : Clinical and Laboratory Research·Wioleta M ZelekB Paul Morgan
Jan 29, 2020·Cell Biochemistry and Function·Mahmoud Abdel-LatifBasant M Morsy
Oct 3, 2006·Current Opinion in Anaesthesiology·W T McBride, S J McBride
Sep 17, 2020·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Graciosa Q TeixeiraCornelia Neidlinger-Wilke
Apr 20, 2006·American Journal of Physiology. Renal Physiology·Kristin J BergslandFredric L Coe
Dec 12, 2020·Journal of Nephrology·Bernardo FariaFelix Poppelaars
Mar 2, 2021·Frontiers in Immunology·Jacob J E KoopmanCees van Kooten
May 1, 2021·Journal of Clinical Medicine·Delia BogdanetPaula M O'Shea

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative Complement Pathway

The Alternative Complement Pathway is part of the innate immune system, and activation generates membrane attack complexes that kill pathogenic cells. Discover the latest research on the Alternative Complement Pathway.