Urinary excretion profile of methiopropamine in mice following intraperitoneal administration: A liquid chromatography-tandem mass spectrometry investigation.

Drug Testing and Analysis
Cristian CamutoFrancesco Botrè

Abstract

We have considered the urinary excretion profile of methiopropamine (MPA), a thiophene ring-based structural analog of methamphetamine with similar stimulant effects, with the aim of selecting the most appropriate marker(s) of intake that may be useful in forensic analysis. For this purpose, in vitro studies were preliminarily performed on human liver microsomes for tracing the phase I metabolic pathways of MPA, preselecting the best candidates as potential target analytes, and designing the optimal experimental strategy. In vivo studies were then conducted on mice, after the intraperitoneal administration of a 10-mg/kg dose. Urine samples were collected every 3 h in the first 9 h and, subsequently, from 24 to 36 h, and stored at -80°C until further analysis. The measurements were performed using a targeted procedure based on liquid/liquid extraction followed by liquid chromatography-tandem mass spectrometry analysis. Our results show that in the time interval 0-9 h after administration, MPA was extensively oxidized mainly to nor-MPA, oxo-MPA, and two hydroxylated metabolites (ie, hydroxy-aryl-methiopropamine and hydroxy-alkyl-methiopropamine). All phase I metabolites underwent phase II metabolism, with the formation of nor-hyd...Continue Reading

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Nov 6, 2018·Drug Testing and Analysis·Monica MazzarinoFrancesco Botrè
May 2, 2020·International Journal of Legal Medicine·Fabio De-GiorgioMatteo Marti

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Citations

Nov 14, 2020·Drug Testing and Analysis·Mario ThevisHans Geyer
Jan 2, 2021·Frontiers in Psychiatry·Massimo di GiannantonioGiovanni Martinotti

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