Urinary excretion profiles of 11-nor-9-carboxy-delta9-tetrahydrocannabinol and 11-hydroxy-delta9-THC: cannabinoid metabolites to creatinine ratio study IV

Forensic Science International : Synergy
Albert D Fraser, David Worth

Abstract

The objective of this study was to compare urinary excretion patterns of two cannabinoid metabolites in subjects with a history of chronic marijuana use. The first metabolite analyzed was nor-9-carboxy-delta9-tetrahydrocannabinol (delta9-THC-COOH), the major urinary cannabinoid metabolite that is pharmacologically inactive. The second metabolite 11-OH-delta9-THC is an active cannabinoid metabolite and is not routinely measured. Urine specimens were collected from four subjects on 12-20 occasions > or = 96 h apart in an uncontrolled clinical setting. Creatinine was analyzed in each urine specimen by the colorimetric modified Jaffé reaction on a SYVA 30R biochemical analyzer. All urine specimens analyzed for 11-OH-delta9-THC had screened positive for cannabinoids with the EMIT II Plus cannabinoids assay (cut-off 50 ng/mL) on a SYVA 30R analyzer and submitted for delta9-THC-COOH confirmation by GC-MS (cut-off concentration 15 ng/mL). Eleven-OH-delta9-THC was measured by GC-MS with a cut-off concentration of 3 ng/mL. Both GC-MS methods for cannabinoid metabolites used deuterated internal standards for quantitative analysis. The mean (range) of urinary delta9-THC-COOH concentration was 1153 ng/mL (78.7-2634) with a cut-off of 15 ng/...Continue Reading

References

Oct 1, 1995·Journal of Analytical Toxicology·M A HuestisE J Cone
Feb 11, 1998·Journal of Forensic Sciences·A D Fraser
Oct 27, 1998·Journal of Analytical Toxicology·M A Huestis, E J Cone
Sep 22, 2001·Forensic Science International : Synergy·A D Fraser
May 14, 2003·Forensic Science International : Synergy·Albert D Fraser, David Worth

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Citations

Jun 27, 2006·British Journal of Sports Medicine·M SaugyJ Dvorak
Apr 1, 2010·Drug Testing and Analysis·Ute MareckWilhelm Schänzer
Aug 23, 2007·Chemistry & Biodiversity·Marilyn A Huestis
Jun 15, 2005·Analytical Chemistry·T A BrettellR Saferstein

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