Urinary Exosomes and Exosomal CCL2 mRNA as Biomarkers of Active Histologic Injury in IgA Nephropathy

The American Journal of Pathology
Ye FengBi-Cheng Liu

Abstract

IgA nephropathy (IgAN) features variable renal pathology and a heterogeneous clinical course. Our aim was to search noninvasive biomarkers from urinary exosomes for IgAN patients; membrane nephropathy and minimal change disease were included as other glomerulopathy controls. Transmission electron microscopy and nanoparticle tracking analysis confirmed the size and morphology characteristic of urinary exosomes. Exosome markers (Alix and CD63) as well as renal cell markers [aquaporin 2 (AQP2) and nephrin] were detected, which indicate the renal origin of urinary exosomes. Exosome excretion was increased markedly in IgAN patients compared with controls and correlated with levels of proteinuria and tubular injury. More important, urinary exosome excretion correlated with greater histologic activity (mesangial hypercellularity, crescents, and endocapillary hypercellularity). Profiling of the inflammation-related mRNA revealed that exosomal chemokine (C-C motif) ligand 2 (CCL2) was up-regulated in IgAN patients. In a validation study, CCL2 was exclusively highly expressed in IgAN patients compared with healthy controls as well as minimal change disease and membrane nephropathy patients. Also, a correlation between exosomal CCL2 and e...Continue Reading

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References

Aug 5, 1998·Nephron·P FreeseG Nyberg
Aug 3, 2002·Nature Reviews. Immunology·Clotilde ThérySebastian Amigorena
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Trairak PisitkunMark A Knepper
Dec 2, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Juhani MyllymäkiJukka Mustonen
Dec 28, 2006·Kidney International·J M MyllymäkiJ T Mustonen
Jun 30, 2012·Nature Cell Biology·James H Hurley, Greg Odorizzi
Aug 16, 2013·American Journal of Physiology. Renal Physiology·Lin-Li LvBi-Cheng Liu
Jan 1, 2012·Journal of Extracellular Vesicles·Olivier G de JongBas W M van Balkom
Oct 23, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lin-Li LvBi-Cheng Liu
May 28, 2014·Nature Reviews. Nephrology·Ian S D Roberts
Jun 5, 2015·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Cristina SoléJosep Ordi-Ros
Mar 31, 2016·Clinical Journal of the American Society of Nephrology : CJASN·Yan-Feng ZhaoHong Zhang
Aug 1, 2016·Journal of Diabetes and Its Complications·Sanaa EissaYasser Soliman
Mar 28, 2017·Kidney International·Hernán TrimarchiUNKNOWN Conference Participants
Jun 19, 2017·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·See Cheng YeoJonathan Barratt
Jul 26, 2017·Journal of the American Society of Nephrology : JASN·Rosanna Coppo, Fernando C Fervenza
Jan 4, 2018·Journal of the American Society of Nephrology : JASN·Lin-Li LvBi-Cheng Liu
Jan 5, 2018·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Pascal Zhongping WeiCheuk Chun Szeto

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Citations

Dec 27, 2018·Journal of Cellular and Molecular Medicine·Lin-Li LvBi-Cheng Liu
Aug 3, 2019·Electrophoresis·Sergio Ayala-MarJosé González-Valdez
Sep 6, 2020·Current Opinion in Nephrology and Hypertension·In O Sun, Soon Hyo Kwon
May 3, 2019·The Korean Journal of Internal Medicine·Soon Hyo Kwon
Jan 9, 2019·Frontiers in Immunology·Artur SłomkaMiroslaw Kornek
Aug 28, 2020·Disease Markers·Christos MasaoutisStamatios Theocharis
Feb 29, 2020·International Journal of Molecular Sciences·Guangbi LiPin-Lan Li
Feb 23, 2020·Frontiers in Pharmacology·Visith Thongboonkerd
Jan 5, 2021·Expert Review of Proteomics·Isabella PanfoliGianluigi Zaza
Oct 31, 2020·Frontiers in Immunology·Haresh SelvaskandanJonathan Barratt
Jan 29, 2021·BioMed Research International·Jialing LiuZhihe Zhao
Feb 24, 2021·Journal of Cell Communication and Signaling·Baichao SunGuangdong Sun
May 3, 2021·Free Radical Biology & Medicine·Diana Karpman, Ashmita Tontanahal
Apr 20, 2021·Kidney Research and Clinical Practice·Sul A LeeTae-Hyun Yoo
Jul 3, 2021·International Journal of Molecular Sciences·Giulia CricrìFederica Collino
Jul 9, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Negin FarzamikiaMohammadreza Ardalan

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