Urinary miRNA-27b-3p and miRNA-1228-3p correlate with the progression of Kidney Fibrosis in Diabetic Nephropathy

Scientific Reports
Francesca ConservaLoreto Gesualdo

Abstract

Diabetic Nephropathy (DN) is a chronic complication of diabetes and the primary cause of end stage renal disease. Differential diagnosis for DN requires invasive histological investigation, thus there is need for non-invasive biomarkers to discriminate among different histological lesions in diabetic patients. With the aim to identify a pattern of differentially expressed miRNAs in kidney biopsies of DN patients, we assayed miRNA expression in kidney biopsies from DN patients, diabetic patients with membranous nephropathy and patients with normal histology. Nine miRNAs were differentially expressed among the three groups, and 2 miRNAs (miR-27b-3p and miR-1228-3p) showed interaction with an ubiquitin-conjugating E2 enzyme variant (UBE2v1). UBE2v1 mediates the formation of lysine 63-linked ubiquitin chains, a mechanism we previously showed as involved in DN kidney fibrosis. Both miRNAs were validated as down-regulated in biopsies and urines of DN patients, possibly affected by DNA methylation. Interestingly, the urinary levels of both miRNAs could also discriminate among different degrees of renal fibrosis. Finally, we showed that the combined urinary expression of both miRNAs was also able to discriminate DN patients from other ...Continue Reading

References

Mar 29, 2002·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Gianna MazzuccoGuido Monga
Dec 25, 2007·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Jianhui ZhouXinyuan Tong
Jun 26, 2013·Acta Diabetologica·Loredana FiorentinoMassimo Federici
Oct 4, 2013·BioMed Research International·Swayam Prakash SrivastavaKeizo Kanasaki
Jan 1, 2014·World Journal of Diabetes·Maria L Gonzalez SuarezAlessia Fornoni
Jun 20, 2014·Wiley Interdisciplinary Reviews. RNA·Chiranjib ChakrabortyGovindasamy Agoramoorthy
Apr 17, 2015·Annals of the New York Academy of Sciences·Mitsuo Kato, Rama Natarajan
Jun 3, 2015·Journal of Cellular Physiology·Jin WangSubrata Sen
Aug 13, 2015·ELife·Vikram AgarwalDavid P Bartel
Dec 29, 2015·European Journal of Clinical Investigation·Michael RudnickiGert Mayer
Apr 14, 2016·European Journal of Heart Failure·Francine Z MarquesDavid M Kaye
Jun 15, 2018·Molecular and Cellular Endocrinology·Taís S AssmannDaisy Crispim

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Citations

Apr 24, 2020·Nephrology·Nisha SinhaSanjeev Puri
Dec 31, 2019·Current Drug Targets·Adele SoltaniAbdolamir Allameh
Feb 25, 2020·Oxidative Medicine and Cellular Longevity·Yang ChenYanrong Lu
Jul 7, 2020·Journal of Diabetes Research·Linhao XuYizhou Xu
Jul 14, 2020·Journal of Diabetes Research·Jiarong LvShizhong Bu
Dec 18, 2020·JCI Insight·Katie L ConnorLaura Denby
Feb 13, 2021·Frontiers in Pharmacology·Yue-Yu GuHui-Yao Lan
Mar 23, 2020·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xin MouWen Hong Liu
Nov 27, 2019·Molecular Metabolism·Haiyan FuDong Zhou
Apr 11, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Manisha J OzaYogesh A Kulkarni
Jun 3, 2021·International Journal of Molecular Sciences·Paola PontrelliLoreto Gesualdo
Oct 31, 2021·Diabetology & Metabolic Syndrome·Mei LinPing Li

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Datasets Mentioned

BETA
GSE51674

Methods Mentioned

BETA
biopsy
ubiquitination
biopsies
PCR
methylation profiling
electron microscopy
Assay
PCRs

Software Mentioned

DIANA
DIANA MicroT - CDS
GeneSpring GX
TargetScan
Adobe Photoshop
R
Roche LC
ImageScope

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