Urinary mutagenicity as a biomarker in workers exposed to benzidine: correlation with urinary metabolites and urothelial DNA adducts

Carcinogenesis
David M DeMariniNathaniel Rothman

Abstract

Urinary mutagenicity has been used in occupational and epidemiological studies for over two decades as a cost-effective, general biomarker of exposure to genotoxic agents. However, few studies have compared urinary mutagenicity to additional biomarkers determined among low- and high-exposed groups. To address this issue, we evaluated the relationship between urinary mutagenicity and other types of biomarkers in a cross-sectional study involving 15 workers exposed to the urinary bladder carcinogen benzidine (BZ, high exposure), 15 workers exposed to BZ-dyes (low exposure), and 13 unexposed controls in Ahmedabad, India. Urinary organics were extracted by C18/methanol and evaluated for mutagenicity in the presence of S9 in the Salmonella strain YG1024, which is a frameshift strain that overproduces acetyltransferase. The results were compared to biomarker data reported recently from the same urine samples (Rothman et al., Proc. Natl Acad. Sci. USA, 93, 5084-5089, 1996) that included a metabolite biomarker (the sum of the urinary levels of BZ + N-acetylbenzidine + N,N'-diacetylbenzidine) and a DNA adduct biomarker [a presumptive N-(3'-phosphodeoxyguanosin-8-yl)-N'-acetylbenzidine (C8dG-ABZ) DNA adduct in exfoliated urothelial cells...Continue Reading

Citations

Oct 8, 1999·Toxicology Letters·V K Bhatnagar, G Talaska
Jul 25, 2009·Journal of Occupational and Environmental Hygiene·Nancy B HopfGlenn Talaska
Apr 23, 2005·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Glenn Talaska, Mustafa Al-Zoughool
Aug 22, 2008·Journal of Occupational Health·Soraya D VarellaEliana A Varanda
Dec 23, 2004·Journal of Occupational Health·Ana FanloEnrique Martínez-Ballarín
Nov 20, 2002·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Gabriele Sabbioni, Christopher R Jones
Feb 14, 2007·Environmental and Molecular Mutagenesis·Sofia PavanelloErminio Clonfero
Dec 2, 2004·Mutation Research·David M DeMarini
Sep 10, 2016·ACS Central Science·Tyler B HughesS Joshua Swamidass
Nov 4, 2020·Occupational and Environmental Medicine·Chieh-Ming WuOlorunfemi Adetona
Sep 4, 2020·Journal of Chemical Information and Modeling·Tyler B HughesS Joshua Swamidass
Sep 22, 2017·Chemical Research in Toxicology·Gabriele Sabbioni

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