Urinary shedding of pathogenic Leptospira in stray dogs and cats, Algiers: A prospective study

PloS One
Sara ZaidiIdir Bitam

Abstract

Leptospirosis is an important worldwide zoonosis. This disease is caused by pathogenic species of the genus Leptospira which are maintained in the environment via chronic renal infection of carrier animals which can be asymptomatic excretors of the organisms in their urines and become a source of infection for humans and other hosts. The prevalence of animal leptospirosis in Algiers, Algeria, is unknown. Real-time PCR and standard PCR and sequencing were used to detect pathogenic Leptospira organisms in the urines of stray dogs and cats in Algiers. In the presence of appropriate controls, none of the 107 cat urine samples were positive while 5/104 (4.8%) canine urine samples (asymptomatic mixed-breed dogs, three females and two males) were positive in two real-time PCR assays targeting the rrs and hsp genes. The positivity of these samples was confirmed by partial PCR-sequencing of the rpoB gene which yielded 100% sequence similarity with Leptospira interrogans reference sequence. In this study, L. interrogans prevalence was significantly higher in dogs aged < one year (16.46% - 29.41%) than in adults (0%) (P value = 0.0001) and then in the overall dog population (2.68% - 4.8%) (P = 0.0007). These results suggest that dogs are ...Continue Reading

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Citations

Dec 24, 2018·Transboundary and Emerging Diseases·Fabienne SprißlerKatrin Hartmann
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Methods Mentioned

BETA
PCR
electrophoresis
PCRs

Software Mentioned

HURBAL
BLAST
MEDCALC®
Basic Local Alignment Search Tool ( BLAST )
ChromasPro

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