Jun 11, 2005

Urinary uronate and sulfated glycosaminoglycan levels: markers for interstitial cystitis severity

The Journal of Urology
Vinata B LokeshwarAngelo E Gousse

Abstract

Urologists frequently rely on symptom and problem indexes to monitor patients with interstitial cystitis (IC). Uronic acid is a component of most glycosaminoglycans (GAGs), which is a protective bladder urothelium coating. We evaluated whether urinary uronate and sulfated GAG levels correlate with IC severity and we characterized urinary GAG species. Urine samples, and a completed O'Leary-Sant IC symptom and problem index questionnaire were obtained from 37 patients with IC and 14 normal individuals. Patients with IC were in group 1-1 or 2 indexes less than 50% the maximum score or group 2-each index 50% or greater the maximum score. All patients fulfilled National Institute of Diabetes and Digestive and Kidney Diseases criteria except glomerulations. Urinary uronate was fractionated using cetyltrimethylammonium bromide (CETAB). Uronate and sulfated GAG levels in urine, CETAB precipitates and CETAB supernatants were measured by the Bitter and Muir, and Farndale assays, respectively, and normalized to creatinine in microg/mg creatinine. GAG species were analyzed by agarose gel electrophoresis. Mean urinary uronate levels were increased in group 2 compared with normal and group 1 values regardless of glomerulations and treatment ...Continue Reading

Mentioned in this Paper

Urinalysis
Sephadex G
Urine
Creatinine
Adequan
Dimethyl Sulfoxide
Heparin
Neoplasm of Uncertain or Unknown Behavior of Bladder
Electrophoresis, Agar Gel
Glycosaminoglycans

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