Urine from current smokers induces centrosome aberrations and spindle defects in vitro in nonmalignant human cell lines

Cancer Genetics and Cytogenetics
Ute GabrielAlice Fabarius


Tobacco smoke containing numerous derived chemical carcinogens is the main risk factor for urothelial carcinoma. These carcinogens can induce DNA damage leading to chromosomal instability, which plays a fundamental role in urothelial carcinogenesis. Possible mechanisms could be centrosomal aberrations, which cause defective spindles and may be responsible for genetic instability. We evaluated the effect of urine from never smokers (NS) and current smokers (CS) in concentrations of 0 to 50% on cell proliferation, chromosomes, centrosomes, and the spindle status of normal human dermal fibroblasts and normal human urothelial cells (UROtsa). After 2 weeks of urine treatment, cell cultures were analyzed by centrosome and spindle immunostaining and conventional cytogenetics. Effects were compared to results of untreated controls. Analysis of normal human dermal fibroblasts and UROtsa cells revealed that urine from CS induced higher values of centrosome aberrations in a dose-dependent and cell line-independent manner when compared to cultures treated with urine from NS and untreated controls. Centrosomal alterations correlated with spindle defects and an increase of sporadic chromosomal aberrations. The observations suggest a causativ...Continue Reading


Jan 1, 1990·Journal of Pharmaceutical and Biomedical Analysis·M B MattammalB B Davis
Oct 15, 1989·International Journal of Cancer. Journal International Du Cancer·J ClavelD Hemon
Jan 1, 1995·Urological Research·J L PetzoldtJ R Masters
Mar 30, 2000·International Journal of Cancer. Journal International Du Cancer·P BrennanP Boffetta
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·A BardelliC Lengauer
May 15, 2001·International Journal of Cancer. Journal International Du Cancer·I Fadl-ElmulaC Larsson
Sep 21, 2001·Environmental Health Perspectives·M R RossiD A Sens
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Franck BorelRobert L Margolis
May 10, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C J SmithC Hansch
Jun 13, 2003·Acta Oncologica·Lúcio SantosBegoña Criado
Jul 17, 2003·International Journal of Cancer. Journal International Du Cancer·Feng JiangRuth L Katz
Sep 25, 2003·International Journal of Cancer. Journal International Du Cancer·Andreas SchneeweissAlwin Krämer
Dec 14, 2004·Nature Cell Biology·Michael A Lampson, Tarun M Kapoor
Nov 17, 2005·Carcinogenesis·R A CrallanJ Southgate
Mar 25, 2006·International Journal of Cancer. Journal International Du Cancer·Wolfgang A Schulz
Jun 29, 2006·Journal of Analytical Toxicology·Kirsten RiedelAnthony R Tricker
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Margaret A Knowles
Mar 14, 2007·Cancer Genetics and Cytogenetics·Yoshiaki YamamotoKohsuke Sasaki
Sep 26, 2007·International Journal of Cancer. Journal International Du Cancer·Sara GandiniPeter Boyle
Feb 7, 2008·Archives of Toxicology·Andrea R Florl, Wolfgang A Schulz
Jan 14, 2009·Cancer Prevention Research·Trevor M Penning, Caryn Lerman
Mar 20, 2009·BMC Complementary and Alternative Medicine·Mark Barton FrankHsueh-Kung Lin
Apr 25, 2009·Cancer Genetics and Cytogenetics·Yoshiaki YamamotoHideyasu Matsuyama
Sep 18, 2009·Developmental Cell·Meng-Fu Bryan TsouPrasad V Jallepalli
Nov 18, 2009·Journal of the National Cancer Institute·Dalsu BarisDebra T Silverman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Related Papers

Urological Research
J L PetzoldtJ R Masters
The American Journal of Pathology
Marshall J UristCarlos Cordon-Cardo
BJU International
Patrick A KenneyIan C Summerhayes
© 2021 Meta ULC. All rights reserved