Urine is necessary to provoke bladder inflammation in protamine sulfate induced urothelial injury

The Journal of Urology
Roberto SolerValdemar Ortiz

Abstract

The bladder is normally impermeable to possible hostile environmental factors and toxic urinary wastes. Any disruption of the permeability barrier would permit the leakage of urine constituents into the underlying cells layers and subsequent inflammation. Protamine sulfate, which increases urothelial permeability, is used in experimental models of cystitis. We examined whether protamine sulfate alone could cause bladder inflammation or if the association of protamine sulfate and urine is needed for this condition. Female Wistar rats (Center for the Development of Experimental Models for Medicine and Biology, Federal University of São Paulo, São Paulo, Brazil) had the bladder catheterized and instilled with protamine sulfate (10 mg) or sterile saline for 30 minutes. To exclude urine other groups of rats underwent bilateral nephrectomy and the same procedure was used. One day after instillation the bladders were removed for histopathology. Edema and vascular congestion were graded from 0-none to 3-severe. Polymorphonuclear and mast cells were counted. The Kruskal-Wallis test was performed for statistical analysis. Intravesical instillation of protamine sulfate in nonnephrectomized rats led to inflammation, in contrast to findings...Continue Reading

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Citations

Apr 5, 2013·Histochemistry and Cell Biology·Andreja ErmanPeter Veranič
Apr 17, 2014·BioMed Research International·Eric J GonzalezMargaret A Vizzard
Nov 24, 2012·The Journal of Urology·Kristina M StemlerIndira U Mysorekar
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Mar 18, 2011·Neurourology and Urodynamics·Roberto SolerJames Koudywilliam
Feb 6, 2013·International Journal of Urology : Official Journal of the Japanese Urological Association·Yi-Song LvWei-Ping Hao
Jun 11, 2011·Neurourology and Urodynamics·Karl-Erik AnderssonClaudius Füllhase
Nov 30, 2013·International Journal of Urology : Official Journal of the Japanese Urological Association·Yi Song LvJian Huang
Sep 14, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Qing YangHsueh-Kung Lin
Jul 1, 2015·Antimicrobial Agents and Chemotherapy·Patrick O WagersDavid A Hunstad
Feb 26, 2016·American Journal of Physiology. Renal Physiology·Robert E HurstBeverley Greenwood-Van Meerveld

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