Urine Metabolomics for Renal Cell Carcinoma (RCC) Prediction: Tryptophan Metabolism as an Important Pathway in RCC

Frontiers in Oncology
Xiaoyan LiuYushi Zhang

Abstract

Renal cell carcinoma (RCC) is the second most lethal urinary cancer. RCC is frequently asymptomatic and it is already metastatic at diagnosis. There is an urgent necessity for RCC specific biomarkers selection for diagnostic and prognostic purposes. In present study, we applied liquid chromatography-mass spectrometry (LC-MS) based metabolomics to analyze urine samples of 100 RCC, 34 benign kidney tumors and 129 healthy controls. Differential metabolites were analyzed to investigate if urine metabolites could differentiate RCC from non-RCC. A panel consisting of 9 metabolites showed the best predictive ability for RCC from the health controls with an area under the curve (AUC) values of 0.905 for the training dataset and 0.885 for the validation dataset. Separation was observed between the RCC and benign samples with an AUC of 0.816. RCC clinical stages (T1 and T2 vs. T3 and T4) could be separated using a panel of urine metabolites with an AUC of 0.813. One metabolite, N-formylkynurenine, was discovered to have potential value for RCC diagnosis from non-RCC subjects with an AUC of 0.808. Pathway enrichment analysis indicated that tryptophan metabolism was an important pathway in RCC. Our data concluded that urine metabolomics co...Continue Reading

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Citations

Jul 8, 2020·International Journal of Environmental Research and Public Health·Joanna MatysiakJan Matysiak
Oct 12, 2020·Archives of Biochemistry and Biophysics·Yuan-Yuan ChenYing-Yong Zhao
Sep 19, 2020·The Cancer Journal·Christian R HoernerAlice C Fan
Jun 24, 2021·Journal of Proteome Research·Olatomiwa O BifarinArthur S Edison

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Methods Mentioned

BETA
surgical resection
nuclear magnetic resonance
PCA

Software Mentioned

Progenesis QI
SIMCA
DA
OPLS
Mummichog
MetaboAnalyst

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