Urine proteome analysis by C18 plate-matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis and prediction of diabetic nephropathy

PloS One
Chao-Jung ChenFuu-Jen Tsai

Abstract

Diabetic nephropathy (DN) is one of the most common complications in diabetic patients. New noninvasive markers are still needed for the early detection of DN before identifiable alternations in kidney function or urine albumin excretion occurs. A C18 plate and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were used to compare the urinary protein profiles of 238 subjects from the following 4 groups: patients with type 2 diabetic (T2D) with microalbuminuria, patients with DM without micro- or macroalbuminuria, patients with micro- or macroalbuminuria due to nondiabetic disease, and healthy controls. β2-microglobulin (B2M) and Clara-cell protein (CC16) were found to be highly released in the urine of patients with proteinuria due to nondiabetic or diabetic diseases. In differentiating nephropathy from healthy subject, the B2M and CC16 markers have a combined sensitivity and specificity of 77.3% and 91.8%, respectively. In distinguishing T2D with microalbuminuria from T2D patients, the combined markers have sensitivity and specificity of 66% and 73%, respectively. The predictive ability of B2M and CC16 for early renal functional decline (ERFD) was validated in 125 T2D patients with a f...Continue Reading

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Citations

Dec 14, 2019·Therapeutic Advances in Endocrinology and Metabolism·Yongzhang QinBaocheng Chang
Apr 16, 2020·International Journal of Molecular Sciences·Lucas Opazo-RíosJesús Egido
May 1, 2021·Proteomics. Clinical Applications·Shu YeShichun Du

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Methods Mentioned

BETA
solid-phase chromatography
chip
chips
protein assay
ELISA

Software Mentioned

Mascot
SPSS
DataAnalysis
SigmaPlot

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