Urine proteomic analysis differentiates cholangiocarcinoma from primary sclerosing cholangitis and other benign biliary disorders

Gut
J MetzgerTim O Lankisch

Abstract

Diagnosis and curative treatment of cholangiocarcinoma (CC) often comes too late due to the lack of reliable tumour markers especially in patients with primary sclerosing cholangitis (PSC). The authors recently introduced bile proteomic analysis for CC diagnosis. Nevertheless, bile collection depends on invasive endoscopic retrograde cholangiography. The authors therefore evaluated urine proteomic analysis for non-invasive CC diagnosis. Using capillary electrophoresis mass spectrometry the authors established a CC-specific peptide marker model based on the distribution of 42 peptides in 14 CC, 13 PSC and 14 benign biliary disorder (BBD) patients. In cross-sectional validation of 123 patients, the urine peptide marker model correctly classified 35 of 42 CC patients and 64 of 81 PSC and BBD patients with an area under the curve value of 0.87 (95% CI 0.80 to 0.92, p=0.0001, 83% sensitivity, 79% specificity). Evaluation of 101 normal controls resulted in 86% specificity. All 10 patients with CC on top of PSC were correctly classified. The majority of sequence-identified peptides are fragments of interstitial collagens with some of them also detected in blood indicating their extra-renal origin. Immunostaining of liver sections for ...Continue Reading

Citations

Jul 3, 2013·Lancet·Gideon M HirschfieldDavid H Adams
Mar 23, 2013·Disease Models & Mechanisms·Abigail ZabronShahid A Khan
Mar 29, 2014·Analytical and Bioanalytical Chemistry·Zhiyong Yang, Jonathan V Sweedler
Apr 2, 2014·Current Opinion in Gastroenterology·John E EatonJayant A Talwalkar
Apr 1, 2014·Clinical and Translational Medicine·Maria FrantziAgnieszka Latosinska
Jan 1, 2014·Journal of Proteomics·Maria FrantziAxel S Merseburger
Nov 21, 2014·Bioanalysis·Szymon FilipHarald Mischak
Dec 24, 2015·Expert Review of Molecular Diagnostics·Samir HanashEdwin J Ostrin
Aug 21, 2013·Scandinavian Journal of Gastroenterology·Henrike LenzenTim O Lankisch
Jan 11, 2014·Expert Review of Proteomics·Julie KleinJoost P Schanstra
Aug 27, 2013·Expert Opinion on Medical Diagnostics·James R A SkipworthStephen P Pereira
Jan 16, 2016·Experimental Eye Research·Matthias NoblMichael J Koss
May 24, 2014·Mass Spectrometry Reviews·Anthonius A M HeemskerkOleg A Mayboroda
Aug 12, 2014·Journal of Hepatology·Per SangfeltFredrik Rorsman
Feb 21, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Michael P Manns
Mar 13, 2014·Journal of Hepato-biliary-pancreatic Sciences·Atit SilsirivanitChaisiri Wongkham
Jun 9, 2015·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Sumera RizviGregory J Gores
May 13, 2015·Best Practice & Research. Clinical Gastroenterology·Annika Bergquist, Erik von Seth
May 13, 2015·Best Practice & Research. Clinical Gastroenterology·Torsten Voigtländer, Tim O Lankisch
Apr 23, 2013·Journal of Hepatology·Tom H Karlsen, Kirsten Muri Boberg
Sep 23, 2014·Journal of Hepatology·Jessica K DysonDavid E J Jones
Mar 5, 2015·Proteomics. Clinical Applications·Judy GopalHui-Fen Wu
May 20, 2015·British Medical Bulletin·Kate D Williamson, Roger W Chapman
Feb 25, 2015·Digestive Diseases and Sciences·Udayakumar NavaneethanRaed A Dweik
Mar 20, 2015·Journal of Carcinogenesis·Yezaz Ahmed GhouriBoris Blechacz
Jul 1, 2016·PloS One·Michael ReichMichael J Koss
Jan 20, 2016·Pancreas·Bastian SchönemeierTim O Lankisch
Aug 17, 2016·Kidney International·Julie KleinJoost P Schanstra
Dec 21, 2016·Nature Reviews. Clinical Oncology·Eric P van der StokErnst J Kuipers
May 2, 2013·Therapeutic Advances in Chronic Disease·Palak J Trivedi, Gideon M Hirschfield
Mar 29, 2018·Scientific Reports·Iwona BelczackaVera Jankowski
Mar 25, 2017·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ru WeiLinda C Burkly
Apr 12, 2018·The American Journal of Tropical Medicine and Hygiene·Gyem TsheringKesara Na-Bangchang
Jun 12, 2018·Mass Spectrometry Reviews·Iwona BelczackaMaria Frantzi
May 29, 2018·Digestive Diseases·Sven H LoosenTom Luedde
Oct 6, 2017·Proteomics. Clinical Applications·Agnieszka LatosinskaHarald Mischak

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