Urine sampling for real-time polymerase chain reaction based diagnosis of canine leishmaniasis

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Laura MannaAngelo Elio Gravino

Abstract

A real-time polymerase chain reaction (PCR) assay was used for quantifying Leishmania infantum DNA in urine samples from naturally infected dogs. Forty-one infected dogs were divided into 3 groups: 22 dogs showing only cutaneous signs (group 1), 12 dogs showing hematuria (group 2), and 7 dogs affected by severe nephropathy (group 3). Groups 2 and 3 dogs showed altered laboratory parameters related to an impairment of renal function. The real-time PCR analysis showed higher levels of Leishmania DNA in the lymph node aspirates from all groups of infected dogs versus those measured in their blood or urine. Interestingly, urine samples from dogs belonging to groups 2 and 3 contained a higher Leishmania DNA load than that detected in their blood. This finding suggests that a real-time PCR analysis of urine from infected dogs could be a useful and noninvasive tool for monitoring the severity of leishmaniasis.

References

Dec 1, 1992·Veterinary Parasitology·C G NietoE Redondo
Oct 1, 1990·Experimental Parasitology·M R RodgersD F Wirth
Jul 1, 1985·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·M DutraH Rocha
Oct 1, 1996·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·R LopezJ M Molleda
Dec 13, 2000·International Journal for Parasitology·R W Ashford
Jun 28, 2001·Clinical and Diagnostic Laboratory Immunology·S BretagneM Deniau
Aug 2, 2002·The Lancet Infectious Diseases·Philippe J GuerinAnthony D M Bryceson
Jun 14, 2003·Revista Da Sociedade Brasileira De Medicina Tropical·Natalino Salgado FilhoJackson M L Costa
Sep 12, 2003·Diagnostic Microbiology and Infectious Disease·Gabriele SchönianCharles L Jaffe
Apr 30, 2004·The Journal of Infectious Diseases·Dalit Strauss-AyaliGad Baneth
Nov 6, 2004·Journal of Clinical Microbiology·Charles MaryHenri Dumon
Aug 22, 2006·Veterinary Parasitology·Laura MannaAngelo Elio Gravino

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Citations

Oct 2, 2012·Parasites & Vectors·Laura MannaAngelo Elio Gravino
Apr 5, 2011·The American Journal of Tropical Medicine and Hygiene·Nicolas VelandAndrea K Boggild
Apr 7, 2009·Trends in Parasitology·Domenico OtrantoEdward B Breitschwerdt
Nov 21, 2008·Veterinary Parasitology·Felicitat TodolíAlhelí Rodríguez-Cortés
Sep 27, 2008·The Veterinary Journal·Laura MannaAngelo Elio Gravino
Oct 26, 2016·Journal of Microbiological Methods·Rômulo Pessoa-E-SilvaMilena de Paiva-Cavalcanti
Apr 25, 2015·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Ivete Lopes de MendonçaLeucio Camara Alves
Oct 18, 2020·Veterinary and Comparative Oncology·Joaquim HenriquesMargarida Alves

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Datasets Mentioned

BETA
AF291093

Methods Mentioned

BETA
PCR
biopsy
electrophoresis

Software Mentioned

GraphPad InStat

Related Concepts