Urineless estimation of glomerular filtration rate and renal plasma flow in the rat

Journal of Pharmacological and Toxicological Methods
C RönnhedhL E Mather

Abstract

A simple method to perform serial renal clearance studies without urine collection in rats is described. This was applied to nonradiolabelled para-aminohippurate sodium (PAH) and iothalamate sodium (IOT) which were used respectively to estimate renal plasma flow (RPF) and glomerular filtration rate (GFR). Under isoflurane-anesthesia the jugular vein and carotid artery of Fischer 344 rats were cannulated, and a loading dose followed by a continuous infusion of PAH and IOT was administered. Steady state was reached after 30 min, and four arterial blood samples were collected over the next 30 min for analysis by HPLC (day 0). The clearances of PAH and IOT were calculated according to Fick's principle as estimates of RPF and GFR from the ratio of the infusion rates to the median solute concentrations. Three days later the femoral artery and vein were cannulated, and the same study was repeated (day 3). There was no significant difference in renal clearances of PAH and IOT between days 0 and 3. The described method gives values that compare well with others in the literature based on other methods and presents an accurate and simple way of detecting changes in renal function before and after a potentially nephrotoxic treatment regimen.

References

Nov 1, 1990·Kidney International·N H LameireM A Waterloos
Jan 1, 1985·Kidney International·B OdlindB Lindström
Jun 1, 1993·Journal of Pharmacokinetics and Biopharmaceutics·S BjörkmanW F Ebling
Jan 1, 1993·Nephron·F GinevriG M Ghiggeri

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Citations

Jul 13, 2007·Acta Cirúrgica Brasileira·Marco Aurelio MarangoniYara Marcondes Machado Castiglia
Dec 11, 2007·Renal Failure·Luis Antonio Dos Santos DiegoYara Marcondes Machado Castiglia
Mar 18, 2000·Hypertension·T A KotchenR J Roman
Mar 11, 2006·Renal Failure·Marcello de Souza SilvaMaria Luiza Cassetari
May 19, 1998·Anesthesia and Analgesia·M JaquenodI Power
Oct 8, 1999·Anesthesia and Analgesia·H KimL E Mather
Mar 15, 2018·Pain Research and Treatment·Mariana TakakuYara Marcondes Machado Castiglia

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