Urocortin-2 Prevents Dysregulation of Ca2+ Homeostasis and Improves Early Cardiac Remodeling After Ischemia and Reperfusion

Frontiers in Physiology
Alejandro Domínguez-RodríguezTarik Smani

Abstract

Aims: Urocortin-2 (Ucn-2) is a potent cardioprotector against Ischemia and Reperfusion (I/R) injuries. However, little is known about its role in the regulation of intracellular Ca2+ concentration ([Ca2+]i) under I/R. Here, we examined whether the addition of Ucn-2 in reperfusion promotes cardioprotection focusing on ([Ca2+]i handling. Methods and Results: Cardiac Wistar rat model of I/R was induced by transient ligation of the left coronary artery and experiments were conducted 1 week after surgery in tissue and adult cardiomyocytes isolated from risk and remote zones. We observed that I/R promoted significant alteration in cardiac contractility as well as an increase in hypertrophy and fibrosis in both zones. The study of confocal [Ca2+]i imaging in adult cardiomyocytes revealed that I/R decreased the amplitude of [Ca2+]i transient and cardiomyocytes contraction in risk and remote zones. Interestingly, intravenous infusion of Ucn-2 before heart's reperfusion recovered significantly cardiac contractility and prevented fibrosis, but it didn't affect cardiac hypertrophy. Moreover, Ucn-2 recovered the amplitude of [Ca2+]i transient and modulated the expression of several proteins related to [Ca2+]i homeostasis, such as TRPC5 and ...Continue Reading

References

Jun 1, 1983·Journal of the American College of Cardiology·S A RubinH J Swan
Jan 12, 2000·Molecular and Cellular Endocrinology·B K BrarD S Latchman
Feb 7, 2002·Trends in Pharmacological Sciences·Frank M Dautzenberg, Richard L Hauger
Aug 16, 2002·Heart·Flavio Ribichini, William Wijns
Feb 8, 2005·Biochemical and Biophysical Research Communications·Anastasios ChanalarisDavid S Latchman
Mar 28, 2007·Molecular and Cellular Biochemistry·Ronald B DriesenMarcel Borgers
Jan 9, 2008·International Journal of Cardiology·Phitsanu BoonprasertNipon Chattipakorn
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yanhong LiaoLutz Birnbaumer
Jul 16, 2008·Methods : a Companion to Methods in Enzymology·Ola SöderbergUlf Landegren
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Eva van RooijEric N Olson
May 13, 2009·Heart Failure Reviews·Stephan E LehnartGerd Hasenfuss
May 23, 2009·Cardiovascular Research·Eva Calderón-SanchezTarik Smani
Apr 15, 2010·Pflügers Archiv : European journal of physiology·Ching-On WongXiaoqiang Yao
Nov 26, 2010·Clinical and Experimental Pharmacology & Physiology·Tarik SmaniAntonio Ordóñez
Dec 24, 2011·The American Journal of Pathology·Julie Reutenauer-PatteOlivier M Dorchies
Apr 14, 2012·Cardiovascular Research·David Garcia-DoradoHans Michael Piper
Oct 24, 2012·Nature Reviews. Cardiology·Changwon KhoRoger J Hajjar
Jun 26, 2014·Handbook of Experimental Pharmacology·Shunichi ShimizuYasuo Mori
May 6, 2015·Biochimica Et Biophysica Acta·Tarik SmaniJuan A Rosado
Jul 30, 2015·Journal of Molecular and Cellular Cardiology·Alejandro Domínguez-RodríguezJean-Pierre Benitah
Jun 9, 2016·British Journal of Clinical Pharmacology·Colin G StirratDavid E Newby
Jun 22, 2016·Cell and Tissue Research·Virpi Talman, Heikki Ruskoaho
Feb 15, 2017·Current Atherosclerosis Reports·Fabrizio MontecuccoFederico Carbone
Sep 14, 2017·Advances in Experimental Medicine and Biology·Petra Eder

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Citations

Sep 13, 2019·Cardiovascular Drugs and Therapy·Cláudia Monteiro-PintoCarmen Brás-Silva
Feb 23, 2020·Cells·Takuro Numaga-Tomita, Motohiro Nishida
Mar 19, 2019·Frontiers in Physiology·Debora FalcónTarik Smani
Jul 3, 2019·Dose-response : a Publication of International Hormesis Society·Li-Zhen Yang, Ying Chen
Oct 6, 2020·Frontiers in Physiology·Alexandra NjegicElizabeth J Cartwright
Nov 5, 2019·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dimosthenis GiamouridisH Kirk Hammond
Oct 30, 2020·Frontiers in Cell and Developmental Biology·Rui LuoJessica Sabourin
Sep 10, 2021·Annual Review of Pharmacology and Toxicology·Robin S BonPiruthivi Sukumar

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Methods Mentioned

BETA
Fluorescence
transfection
Assay
nucleotide exchange

Software Mentioned

Expression Suite
Exelis
Elements
Duolink ImageTool
IDL
NIS
ImageJ

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