Uroepithelial cells can directly respond to Mycobacterium bovis bacillus Calmette-Guérin through Toll-like receptor signalling

BJU International
Jun MiyazakiHideyuki Akaza

Abstract

To investigate, in a human urinary tract cell line, the interaction of Toll-like receptor (TLR) signals with cytoplasmic adapter proteins MyD88 and bacillus Calmette-Guérin (BCG), and evaluate the epithelial cytokine response to BCG infection. Intravesical BCG therapy is effective against carcinoma in situ and as prophylaxis for recurrence, but although immunological mechanisms have been assumed, the mechanisms of the antitumour effects of BCG have not been completely elucidated. The cell line was first screened for TLR expression by reverse transcription-polymerase chain reaction (RT-PCR). Total RNA was isolated from a human urinary cell line, Hu35E6E7, and cDNA synthesised. PCR was used to measure the gene expression of TLR-2, -3, -4, -5, -9, MyD88, MD-2, CD14 and interleukin-8 and -6. The Hu35E6E7 cell line was cultured in keratinocyte serum-free medium, and BCG was added to the cell culture. After Hu35E6E7 cells were stimulated by BCG for various periods, the total RNA of the cells was extracted. Quantitative real-time PCR was conducted for MyD88 using appropriate probes, and the expression of MyD88 analysed. The cell supernatant was collected, and the levels of interferon-gamma, tumour necrosis factor-alpha, interleukin-2,...Continue Reading

References

Jan 1, 1992·European Urology·T L Ratliff
Feb 27, 1999·Current Opinion in Microbiology·C SvanborgM Hedlund
Dec 29, 2000·Nature·H HemmiS Akira
Sep 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·F TakeshitaD M Klinman
Apr 26, 2002·International Journal of Urology : Official Journal of the Japanese Urological Association·Noriko IkedaHideyuki Akaza
Nov 15, 2002·Molecular Immunology·Sean Diehl, Mercedes Rincón
Jan 14, 2003·Annual Review of Immunology·Kiyoshi TakedaShizuo Akira
Mar 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pierre-Yves BochudAlan Aderem
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Joel D SchillingScott J Hultgren
Aug 7, 2003·Current Opinion in Pharmacology·Luke A J O'Neill
May 25, 2004·Infection and Immunity·Patrik SamuelssonCatharina Svanborg

❮ Previous
Next ❯

Citations

Jan 16, 2007·European Urology·Salvatore SiracusanoGiuliano Zabucchi
Nov 16, 2007·International Journal of Immunogenetics·Y TabelS Mir
Aug 28, 2014·Frontiers in Immunology·Ryan McClure, Paola Massari
Aug 28, 2013·Nature Reviews. Urology·Hélène LaRueYves Fradet
Aug 27, 2019·Scandinavian Journal of Immunology·Solmaz Ohadian Moghadam, Mohammad Reza Nowroozi
Aug 14, 2019·Applied Microbiology and Biotechnology·Martha Lucia Ruiz BenitezFabiana Kömmling Seixas
Jun 20, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jiansong HanQiaoli Wu
Feb 5, 2008·Current Opinion in Microbiology·Jeongmin Song, Soman N Abraham

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.