Uroguanylin: gene structure, expression, processing as a peptide hormone, and co-storage with somatostatin in gastrointestinal D-cells

Regulatory Peptides
H J MägertW G Forssmann

Abstract

Guanylin/GCAP-I and uroguanylin/GCAP-II are two structurally related peptides which play an important role in the regulation of water/electrolyte balance within the gut. In order to enable the investigation and comparison of both peptide hormones at the genomic level, we decided to clone the corresponding genes. The human gene for guanylin/GCAP-I and its 5'-flanking region have been described recently. Here, we report the three exon/two intron structure of the human uroguanylin/GCAP-II gene and its localization on chromosome 1 p35-34, as determined by radiation hybrid mapping. Together with data obtained for the guanylin/GCAP-I gene we show that these genes are localized in the same chromosomal area with other guanlyl cyclase-activating peptides like ANP etc. Northern hybridization revealed that the expression of the uroguanylin/GCAP-II gene is highest in the intestinal mucosa, especially in the ileum and colon. By means of polymerase chain reaction (PCR), an expression was also observed in the stomach where no guanylin/GCAP-I expression is detectable. Using immunohistochemical methods, uroguanylin/GCAP-II immunoreactive material was distinctly localized in D-type gastric and intestinal endocrine cells. Although the comparable ...Continue Reading

References

Aug 1, 1992·Molecular and Cellular Biology·R L WidomS K Karathanasis
Oct 19, 1992·FEBS Letters·R C WiegandM G Currie
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M G CurrieC E Smith
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·F J de SauvageD V Goeddel
Oct 16, 1989·Biochemical and Biophysical Research Communications·B GiglioniS Ottolenghi
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y S Lin, M R Green
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·U SträhleG Schütz
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C Moskaluk, D Bastia
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A DornD Mathis
Sep 11, 1987·Cell·A DornD Mathis
Jan 22, 1982·Nucleic Acids Research·S M Mount
Dec 1, 1995·Gastroenterology·M B Cohen
Mar 25, 1995·Nucleic Acids Research·P D SiebertS A Lukyanov
Apr 26, 1995·Biochemical and Biophysical Research Communications·M B CohenM G Currie
May 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L R Forte, M G Currie
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·O HillH J Mägert
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·H J MägertW G Forssmann
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F K HamraC E Smith
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·K FerreriM Montminy
May 1, 1994·Nature Genetics·M A WalterP N Goodfellow
Mar 21, 1994·FEBS Letters·M KuhnW G Forssmann
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y CetinW G Forssmann
Oct 29, 1993·Biochemical and Biophysical Research Communications·L G LewisM B Cohen
Jul 25, 1996·Biochemical and Biophysical Research Communications·A PardigolW G Forssmann
Oct 25, 1996·Science·G D SchulerT J Hudson

❮ Previous
Next ❯

Citations

Jun 14, 2005·Pflügers Archiv : European journal of physiology·Aleksandra Sindić, Eberhard Schlatter
Mar 30, 2001·European Journal of Biochemistry·N RoyS S Visweswariah
Dec 21, 2002·Protein Science : a Publication of the Protein Society·Alexander KrauseKnut Adermann
Jun 9, 2000·Annual Review of Physiology·L R ForteR H Freeman
Jan 1, 2013·ISRN Nephrology·Aleksandra Sindic
Dec 5, 2006·Current Opinion in Nephrology and Hypertension·Aleksandra Sindić, Eberhard Schlatter
Dec 4, 2012·Kidney International·Thomas Mueller, Benjamin Dieplinger
Sep 22, 2006·Cell Proliferation·I R Radford, P N Lobachevsky
Apr 6, 2016·Cell and Tissue Research·Øystein BrennaBjörn I Gustafsson
May 2, 2015·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A JarmużJ Fichna
Sep 6, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Gerhard KaarThomas Mueller
Jul 27, 1999·The Journal of Biological Chemistry·H J MägertW G Forssmann
Feb 9, 2000·American Journal of Physiology. Renal Physiology·L R ForteW J Krause
Apr 2, 2002·World Journal of Gastroenterology : WJG·Feng-Peng SunYong-Li Yao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.