Urokinase-type plasminogen activator plays essential roles in macrophage chemotaxis and skeletal muscle regeneration

The Journal of Immunology : Official Journal of the American Association of Immunologists
Scott C BryerTimothy J Koh

Abstract

Although macrophages are thought to play important roles in tissue repair, the molecular mechanisms involved remain to be elucidated. Mice deficient in urokinase-type plasminogen activator (uPA-/-) exhibit decreased accumulation of macrophages following muscle injury and severely impaired muscle regeneration. We tested whether macrophage-derived uPA plays essential roles in macrophage chemotaxis and skeletal muscle regeneration. Macrophage uPA was required for chemotaxis, even when invasion through matrix was not necessary. The mechanism by which macrophage uPA promoted chemotaxis was independent of receptor binding but appeared to depend on proteolytic activity. Exogenous uPA restored chemotaxis to uPA-/- macrophages and rescued muscle regeneration in uPA-/- mice. Macrophage depletion in wild-type (WT) mice using clodronate liposomes resulted in impaired muscle regeneration, confirming that macrophages are required for efficient healing. Furthermore, transfer of WT bone marrow cells to uPA-/- mice restored macrophage accumulation and muscle regeneration. In this rescue, transferred WT cells appeared to contribute to IGF-1 expression but did not fuse to regenerating fibers. These data indicate that WT leukocytes, including macr...Continue Reading

References

Apr 1, 1990·Journal of Structural Biology·J M PapadimitriouM D Grounds
Mar 1, 1987·Journal of the Neurological Sciences·A K Gulati
Sep 14, 1994·Journal of Immunological Methods·N Van Rooijen, A Sanders
Apr 1, 1994·Journal of Cellular Physiology·B W FestoffJ Chao
Apr 1, 1994·The Journal of Clinical Investigation·M R GyetkoR G Sitrin
Jan 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J Taipale, J Keski-Oja
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·E R Barton-DavisH L Sweeney
Jul 18, 2000·Experimental Cell Research·B ChazaudG Barlovatz-Meimon
Sep 9, 2000·Current Opinion in Cell Biology·L Ossowski, J A Aguirre-Ghiso
Mar 10, 2001·The American Journal of Pathology·J A BezerraJ L Degen
Apr 20, 2001·Cytokine & Growth Factor Reviews·Y LeJ M Wang
Dec 4, 2002·Nature Reviews. Molecular Cell Biology·Francesco Blasi, Peter Carmeliet
Jan 4, 2003·Nature Reviews. Immunology·Siamon Gordon
Jan 22, 2003·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·M CantiniL Vitiello
Sep 27, 2003·Developmental Biology·Timothy R BrazeltonHelen M Blau
Apr 16, 2004·American Journal of Physiology. Cell Physiology·Brenda A BondesenGrace K Pavlath
Jan 18, 2005·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Takayuki NakamuraHans-Joachim Schäfers
Feb 18, 2005·American Journal of Physiology. Cell Physiology·Timothy J KohThomas H Sisson
Apr 21, 2005·Thrombosis and Haemostasis·Francis J Castellino, Victoria A Ploplis
Feb 9, 2006·Cellular and Molecular Life Sciences : CMLS·P Ragno
Apr 25, 2006·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Sharon D LuikartTheodore R Oegema
Dec 1, 2006·Journal of Leukocyte Biology·Paula K ShiremanLinda M McManus
May 9, 2007·The Journal of Experimental Medicine·Ludovic ArnoldBénédicte Chazaud
Sep 6, 2007·The Journal of Cell Biology·Mònica SuelvesPura Muñoz-Cánoves

❮ Previous
Next ❯

Citations

Jun 4, 2010·Journal of Leukocyte Biology·Deborah J GowDavid A Hume
May 15, 2010·Journal of Neuropathology and Experimental Neurology·Noemí De LunaIsabel Illa
Aug 25, 2012·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Boris ShenkmanUriel Martinowitz
Jun 28, 2014·European Journal of Applied Physiology·Paul A Della GattaJonathan M Peake
Sep 18, 2018·Journal of Functional Morphology and Kinesiology·Thomas F CorbiereTimothy J Koh
Mar 21, 2008·American Journal of Physiology. Cell Physiology·Ming ChengTimothy J Koh
Mar 30, 2010·Journal of Cellular and Molecular Medicine·Adrian DraguRaymund E Horch
Jan 30, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Margaret L NovakTimothy J Koh
Dec 21, 2013·BMC Musculoskeletal Disorders·Claude H CôtéElise Duchesne
Mar 3, 2015·Muscle & Nerve·Maria J Torres-PalsaFrancis X Pizza
Jan 2, 2013·American Journal of Physiology. Endocrinology and Metabolism·Nicolas J PillonAmira Klip
Apr 20, 2016·The Journal of Cell Biology·Joana CapoteMelissa J Spencer
Sep 12, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Milie M FangNorifumi Urao
Aug 31, 2018·International Immunology·Yumiko Oishi, Ichiro Manabe
Mar 14, 2009·Nature Reviews. Immunology·Jeffrey W Pollard
Jun 29, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Margaret L NovakTimothy J Koh
Dec 1, 2012·Nephrology·Maliha A Alikhan, Sharon D Ricardo
Sep 22, 2012·Clinical & Experimental Metastasis·Kasper AlmholtLeif Røge Lund
Nov 24, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chung-Ze WuJin-Shuen Chen
Sep 5, 2020·Immunity, Inflammation and Disease·Andrea F CruzFarzad Esni
Feb 7, 2013·The FEBS Journal·Marielle SaclierBénédicte Chazaud
Mar 13, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew J FleetwoodJohn A Hamilton
Oct 3, 2018·Proceedings of the National Academy of Sciences of the United States of America·Theresa M Raimondo, David J Mooney
Nov 21, 2013·The Journal of Pathology·Margaret L NovakTimothy J Koh
Feb 14, 2015·Journal of Applied Physiology·David W HammersRoger P Farrar
Apr 28, 2020·ANZ Journal of Surgery·Christopher D Barrett, Michael B Yaffe
Oct 5, 2013·The American Journal of Pathology·Margaret L Novak, Timothy J Koh
Jun 22, 2021·Frontiers in Immunology·Noymar Luque-CamposAna María Vega-Letter
Jul 11, 2020·The American Journal of Pathology·Ryan A MartinFrancis X Pizza
Jul 19, 2011·The American Journal of Pathology·Maliha A AlikhanSharon D Ricardo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
Margaret L NovakTimothy J Koh
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Scott C Bryer, Timothy J Koh
American Journal of Physiology. Cell Physiology
Timothy J KohThomas H Sisson
© 2021 Meta ULC. All rights reserved