Abstract
Uropathogenic Escherichia coli (UPEC) is the most common cause of urinary tract infections. In this study, UPEC strains harboring hemolysin A (HlyA) did not induce programmed cell death pathways by the activation of caspases. Instead, the UPEC pore-forming toxin HlyA triggered an increase in mitochondrial Ca2+ levels and manipulated mitochondrial dynamics by causing fragmentation of the mitochondrial network. Alterations in mitochondrial dynamics resulted in severe impairment of mitochondrial functions by loss of membrane potential, increase in reactive oxygen species production, and ATP depletion. Moreover, HlyA caused disruption of plasma membrane integrity that was accompanied by extracellular release of the danger-associated molecules high-mobility group box 1 (HMGB1) and histone 3 (H3). Our results indicate that UPEC induced programmed cell necrosis by irreversibly impairing mitochondrial function. This finding suggests a strategy devised by UPEC at the onset of infection to escape early innate immune response and silently propagate inside host cells.-Lu, Y., Rafiq, A., Zhang, Z., Aslani, F., Fijak, M., Lei, T., Wang, M., Kumar, S., Klug, J., Bergmann, M., Chakraborty, T., Meinhardt, A., Bhushan, S. Uropathogenic Escherich...Continue Reading
References
Jul 1, 1995·Archives of Andrology·G MerinoC Morán
Apr 6, 1999·The American Journal of Medicine·B A Lipsky
Jun 26, 2003·Nihon saikingaku zasshi. Japanese journal of bacteriology·Shingo Yamamoto
Oct 7, 2003·International Journal of Antimicrobial Agents·L EmodyG Nagy
Oct 8, 2004·Cellular Microbiology·Jean-Philippe Nougayrède, Michael S Donnenberg
Mar 1, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·James R JohnsonTorsten Sandberg
Jul 12, 2005·European Urology·G R DohleUNKNOWN EAU Working Group on Male Infertility
Aug 6, 2005·Journal of Clinical Microbiology·Nick J ParhamIan R Henderson
Jun 30, 2006·Cellular Signalling·Driss El KebirJános G Filep
Aug 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Elzbieta BrzuszkiewiczUlrich Dobrindt
Sep 16, 2006·Immunological Reviews·Monika Fijak, Andreas Meinhardt
Apr 9, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sudhanshu BhushanAndreas Meinhardt
Jul 18, 2009·PLoS Pathogens·Catherine L KennedyJulian I Rood
Sep 8, 2009·Microbes and Infection·Linda Böhme, Thomas Rudel
Oct 20, 2009·Journal of Reproductive Immunology·Sudhanshu BhushanAndreas Meinhardt
Feb 23, 2010·PLoS Pathogens·Hua NiuYasuko Rikihisa
Mar 5, 2010·Journal of Biochemical and Molecular Toxicology·Kenneth W YoungPierluigi Nicotera
Jul 20, 2010·Cell Host & Microbe·Mohamed Lamkanfi, Vishva M Dixit
Jul 22, 2010·Nature Reviews. Urology·Greta R Nielubowicz, Harry L T Mobley
Sep 8, 2010·Nature Reviews. Microbiology·Thomas RudelVera Kozjak-Pavlovic
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Fabrizia StavruPascale Cossart
Jul 20, 2011·Nature Chemical Biology·David CarlingSteven J Gamblin
Nov 30, 2011·The Journal of Cell Biology·Hiroshi AshidaChihiro Sasakawa
Dec 14, 2011·PloS One·Sudhanshu BhushanAndreas Meinhardt
Jan 24, 2012·Cell Host & Microbe·Bijaya K Dhakal, Matthew A Mulvey
Jan 24, 2012·Cell·Zhigao WangXiaodong Wang
Apr 13, 2012·IUBMB Life·Jhih-Hang JiangKipros Gabriel
Dec 21, 2012·Future Microbiology·Travis J Wiles, Matthew A Mulvey
Jan 10, 2013·PloS One·Yongning LuAndreas Meinhardt
Feb 26, 2013·Nature Reviews. Immunology·Ravid ShechterMichal Schwartz
Feb 27, 2013·Immunity·Agnieszka KaczmarekDmitri V Krysko
Oct 1, 2013·Molecular and Cellular Endocrinology·Petra ArckAndreas Meinhardt
Feb 20, 2015·Cell Death & Disease·S-J LeeH J Han
Feb 25, 2015·Biochemical Pharmacology·Elodie LobetThierry Arnould
May 23, 2015·Circulation Research·Jason Karch, Jeffery D Molkentin
Jun 27, 2015·Asian Journal of Andrology·Vera MichelAndreas Meinhardt
Nov 26, 2015·Nature Reviews. Microbiology·Pedro EscollCarmen Buchrieser
Dec 2, 2015·The Journal of Infectious Diseases·Zhengguo ZhangSudhanshu Bhushan
Dec 3, 2015·Cold Spring Harbor Perspectives in Biology·Douglas R Green, Fabien Llambi
Dec 15, 2015·PLoS Pathogens·Norberto González-JuarbeCarlos Javier Orihuela
Feb 20, 2016·Journal of Visualized Experiments : JoVE·Sudhanshu BhushanJörg Klug
May 4, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ming WangSudhanshu Bhushan
Citations
Mar 3, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Derek M McKayTimothy Shutt
Mar 15, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ambika M V MurthyMatthew J Sweet
Apr 2, 2019·Preparative Biochemistry & Biotechnology·Vivek VermaManisha Yadav
Jul 30, 2020·Scientific Reports·Vivek VermaManisha Yadav
Jun 25, 2019·Frontiers in Cellular and Infection Microbiology·Leyla MinnullinaAyslu Mardanova
Nov 9, 2018·Antibiotics·Cátia CaneirasAida Duarte
Mar 9, 2021·Frontiers in Immunology·Yongning LuXi Dong