Uroplakin 1b is critical in urinary tract development and urothelial differentiation and homeostasis

Kidney International
Ashley R CarpenterKirk M McHugh

Abstract

Proper development and maintenance of urothelium is critical to its function. Uroplakins are expressed in developing and mature urothelium where they establish plaques associated with the permeability barrier. Their precise functional role in development and disease is unknown. Here, we disrupted Upk1b in vivo where its loss resulted in urothelial plaque disruption in the bladder and kidney. Upk1b(RFP/RFP) bladder urothelium appeared dysplastic with expansion of the progenitor cell markers, Krt14 and Krt5, increased Shh expression, and loss of terminal differentiation markers Krt20 and uroplakins. Upk1b(RFP/RFP) renal urothelium became stratified with altered cellular composition. Upk1b(RFP/RFP) mice developed age-dependent progressive hydronephrosis. Interestingly, 16% of Upk1b(RFP/RFP) mice possessed unilateral duplex kidneys. Our study expands the role of uroplakins, mechanistically links plaque formation to urinary tract development and function, and provides a tantalizing connection between congenital anomalies of the kidney and urinary tract along with functional deficits observed in a variety of urinary tract diseases. Thus, kidney and bladder urothelium are regionally distinct and remain highly plastic, capable of expan...Continue Reading

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Citations

Apr 27, 2016·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Ashley R Carpenter, Kirk M McHugh
Jun 14, 2018·American Journal of Physiology. Renal Physiology·Ashley R JacksonBrian Becknell
Mar 20, 2020·Physiological Reviews·Marianela G DalghiGerard Apodaca
Aug 8, 2020·Metallomics : Integrated Biometal Science·Charles O'DohertyFinbarr O'Sullivan
Jul 20, 2019·American Journal of Physiology. Renal Physiology·Ashley R JacksonBrian Becknell
Jul 11, 2020·Nature Reviews. Urology·Ashley R JacksonBrian Becknell
Feb 23, 2021·Journal of Tissue Engineering·Biao ChenShengwen Li

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