PMID: 42911Dec 1, 1979Paper

Uroporphyrinogen I synthase from human erythrocytes: separation, purification, and properties of isoenzymes

Proceedings of the National Academy of Sciences of the United States of America
K MiyagiC J Watson

Abstract

Uroporphyrinogen I synthase [porphobilinogen ammonia-lyase (polymerizing), EC 4.3.1.8] from human erythrocytes was separated into two active protein peaks (A and B on DEAE-cellulose, by ammonium sulfate fractionation, on Sephadex G-100, and on DEAE-Sephadex A-50 with a NaCl gradient. The final purification was 613 and 743 times for A and B, respectively. The corresponding yields were 2.2 and 3.4% Fraction A was separated further into two (A1 and A2) active protein bands and fraction B into three (B1, B2, and B3) on analytical polyacrylamide disc gel electrophoresis. Bands A1 and A2 were identical with B1 and B2; B3 represented a third isoenzyme. Molecular weights (mean +/- SEM), measured by gel filtration and sodium dodecyl sulfate/polyacrylamide gel electrophoresis, were 38,000 +/- 1000 for B1 and 40,000 +/- 1000 for B2 and B3. Isoelectric focusing on 4% polyacrylamide gel separated both fractions A and B into three active protein bands. Maximal activity of the enzyme was found in gel cuts (5-mm) at pH 5.6 for both fractions A and B.

Citations

Jan 15, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·F W de RooijJ H Wilson
Jan 1, 1980·The International Journal of Biochemistry·K MiyagiC A Pierach
Jan 1, 1980·The International Journal of Biochemistry·Z J PetrykaM K Weimer
Jan 1, 1985·The International Journal of Biochemistry·S A FumagalliA M Batlle
Nov 2, 1988·Biochimica Et Biophysica Acta·M B Mazzetti, J M Tomio
Jul 20, 2002·The International Journal of Biochemistry & Cell Biology·Guillermo NoriegaAdela Ana Juknat
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M H Meisler, M L Carter
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B GrandchampM Goossens
Nov 1, 1989·Scandinavian Journal of Clinical and Laboratory Investigation·L LannfeltP Gellerfors
Aug 12, 2005·International Journal of Cancer. Journal International Du Cancer·Dai NakashimaHideki Tanzawa
Aug 1, 1981·British Journal of Industrial Medicine·T SakaiK Ushio
Nov 16, 2007·Proteins·Luis CunhaRobert J Desnick
May 1, 1992·British Journal of Industrial Medicine·M Chiba, A Shinohara
Jul 9, 2003·British Journal of Haematology·Abas H LaftahTimothy J Peters

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.