Urothelial bladder afferent neurons in the rat are anatomically and neurochemically distinct from non-urothelial afferents

Brain Research
Buffie J Clodfelder-MillerJennifer J DeBerry

Abstract

There is mounting evidence underscoring a role for the urothelium in urinary bladder sensation. Previous functional studies have identified bladder primary afferents with mechanosensitive properties suggesting urothelial innervation and/or communication. The current study identifies a group of urothelium-innervating afferent neurons in rat, and characterizes and compares the properties of these and non-urothelial afferent neuron populations. Lumbosacral (LS) primary afferent neurons were retrogradely labeled using intraparenchymal (IPar) microinjection or intravesical (IVes) infusion of tracer into the bladder. Using these techniques, separate populations of neurons were differentiated by dorsal root ganglion (DRG) somata labeling and dye distribution within the bladder. IPar- and IVes-labeled neurons accounted for 85.0% and 14.4% of labeled L6-S1 neurons (P < .001), respectively, with only 0.6% of neurons labeled by both techniques. Following IVes labeling, dye was contained only within the periurothelial bladder region in contrast to non-urothelial distribution of dye after IPar labeling. Electrophysiological characterization by in situ patch-clamp recordings from whole-mount DRG preparations indicated no significant differen...Continue Reading

References

Apr 1, 1991·The Journal of Urology·C L ParsonsP Stein
Oct 10, 1982·The Journal of Comparative Neurology·C E Hulsebosch, R E Coggeshall
Jun 1, 1996·Journal of Neurophysiology·M S GoldJ D Levine
Jun 17, 2000·Progress in Neurobiology·C KöbbertS Thanos
Oct 12, 2000·Journal of Neurophysiology·V K SheaE R Perl
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·L A BirderM J Caterina
Dec 17, 2009·The American Journal of Pathology·Veronica Sanchez FreireKatia Monastyrskaya
Oct 9, 2013·Annual Review of Cell and Developmental Biology·David Julius
Jan 22, 2014·Nature Communications·Victor MeseguerFélix Viana
Apr 8, 2014·Pain·Jennifer J DeBerryBrian M Davis
Jun 21, 2014·BioMed Research International·Katrina J SmithCatherine McDermott
Apr 22, 2015·The Journal of Pain : Official Journal of the American Pain Society·Jennifer J DeBerryBrian M Davis
Dec 10, 2015·Cell and Tissue Research·Thomas GevaertDirk De Ridder
Jan 29, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Katsumi KadekawaMitsuharu Yoshiyama
Feb 14, 2016·Neurourology and Urodynamics·Anthony KanaiLori Birder
Jun 1, 2017·ENeuro·Nicolas MontalbettiMarcelo D Carattino

❮ Previous
Next ❯

Citations

Nov 2, 2019·Physiological Reviews·Karel TalaveraBernd Nilius
Feb 5, 2021·Nature Reviews. Urology·Matthias VannesteWouter Everaerts
Apr 4, 2021·International Journal of Molecular Sciences·Chung-Kuan WuDer-Cherng Tarng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.