Ursodeoxycholic acid reduces protein levels and nucleation-promoting activity in human gallbladder bile

Gastroenterology
K J Van ErpecumA K Groen

Abstract

Ursodeoxycholic acid prevents gallstone formation in selected patients. The aim of this study was to examine whether decreased concentration and nucleation-promoting activity of various proteins contribute to this beneficial effect. Gallbladder bile of 13 patients with cholesterol gallstones treated with ursodeoxycholic acid (10 mg/kg(-1)/day(-1)) and of 13 untreated patients were compared. Total protein concentration in gallbladder bile (2.8 +/- 0.6 vs. 6.7 +/- 1.3 mg/mL; P=0.008) and concanavalin A-binding fraction (0.16 +/- 0.03 vs. 0.42 +/- 0.07 mg/mL; P=0.003) were strongly decreased by ursodeoxycholic acid therapy. Significant decreases were also found for gallbladder bile alpha1-acid glycoprotein, haptoglobin, immunoglobulin (Ig) A, IgG, gamma-glutamyl transpeptidase, and aminopeptidase N but not for IgM, mucin, or beta-glucuronidase. Decreases were most pronounced for proteins of canalicular membrane origin. Gallbladder bile total protein correlated with cholesterol saturation index (r=0.54; P=0.0047) but not with bile salt hydrophobicity index. Crystallization-promoting activity of the concanavalin A-binding fraction (assessed by nephelometry and microscopic examination) was also significantly decreased by ursodeoxycho...Continue Reading

Citations

Apr 12, 2000·European Journal of Clinical Investigation·P PortincasaG Palasciano
Mar 29, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Niels G VennemanKarel J van Erpecum
Aug 7, 2009·European Journal of Clinical Investigation·I GrattaglianoP Portincasa
Oct 31, 2012·Journal of Gastroenterology and Hepatology·Dong Ki Lee, Sung Ill Jang
May 27, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Niels G VennemanKarel J van Erpecum
Nov 24, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Tadashi Ikegami, Yasushi Matsuzaki
Dec 21, 2004·World Journal of Gastroenterology : WJG·Piero PortincasaGerard P van Berge-Henegouwen
Jul 16, 2002·Biochimica Et Biophysica Acta·Niels G VennemanKarel J van Erpecum
Jun 25, 2003·Journal of Hepatology·Karel J van ErpecumDieter Jüngst
Apr 28, 1999·The Journal of Laboratory and Clinical Medicine·D JüngstS Fischer
May 16, 2003·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·P PortincasaG Palasciano
May 19, 2010·Gastroenterology Clinics of North America·Niels Gerard Venneman, Karel Johannes van Erpecum

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