Ursolic Acid Induces Apoptotic Cell Death Through AIF and Endo G Release Through a Mitochondria-dependent Pathway in NCI-H292 Human Lung Cancer Cells In Vitro

In Vivo
Chiung-Ju ChenJing-Gung Chung

Abstract

Ursolic acid (UA), a triterpene compound present in natural plants, has been shown to induce cytotoxic effects on many human cancer cells through induction of cell-cycle arrest and apoptosis. This study investigated the effects of UA on human lung cancer NCI-H292 cells in vitro. Flow cytometric assay was used to measure the percentage of cell viability, apoptotic cell death by double staining of annexin V and propidium iodide (PI), production of reactive oxygen species (ROS) and Ca2+, and mitochondriaI membrane potential (Ψm). UA-induced chromatin condensation and DNA fragmentation were examined by 4',6-diamidino-2-phenylindole staining and DNA gel electrophoresis, respectively. Western blotting was used to examine the changes of apoptosis-associated protein expression in NCI-H292 cells. UA reduced cell viability and induced apoptotic cell death. UA increased Ca2+ production, reduced Ψm, but did not affect ROS production in NCI-H292 cells. UA increased apoptosis-inducing factor (AIF) and endonuclease G in NCI-H292 cells. Based on these observations, we suggest UA induces apoptotic cell death via AIF and Endo G release through a mitochondria-dependent pathway in NCI-H292 cells.

Citations

Aug 25, 2019·Pathogens·Ines SifaouiIgnacio A Jiménez
Aug 28, 2020·World Journal of Stem Cells·Zi-Jian ZhangYu Wen
Aug 14, 2019·Oncology Letters·Yi PeiXiaojing Zhang
Jul 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ngoc Hong NguyenVan Giau Vo
Jan 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zheng-Yu ChengJing-Gung Chung
Jul 24, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jyoti SinghAbha Meena

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